- ￥960 - 1600
- Solarbio
- 中国 北京
- K200093M
- 2025年07月29日
- WB ; Elisa
- Mouse
- SARS-CoV ; SARS-CoV-2
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant Protein
- 亚型：
IgG1
- 保存条件：
Store at -20℃. Avoid freeze/thaw cycles.
- 克隆性：
单克隆
- 标记物：
Biotin，Cy3，Cy3.5，Cy5，Cy5.5，Cy7，FITC，HRP，RBITC，SAlexa Fluor 350，SAlexa Fluor 405，SAlexa Fluor 488，SAlexa Fluor 532，SAlexa Fluor 555，SAlexa Fluor 568，SAlexa Fluor 594，SAlexa Fluor 633，SAlexa Fluor 640，SAlexa Fluor 647，SAlexa Fluor 660，SAlexa Fluor 680，SAlexa Fluor 750
- 适应物种：
SARS-CoV ; SARS-CoV-2
- 保质期：
Store at -20℃. Avoid freeze/thaw cycles.
- 抗原来源：
Recombinant Protein
- 目录编号：
1B8
- 级别：
实验
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Mouse
- 应用范围：
WB ; Elisa
- 浓度：
WB 1:5000-8000. ELISA 1:5000-50000.
- 靶点：
SARS-CoV/SARS-CoV-2 Nucleocapsid
- 抗体英文名：
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Monoclonal Antibody
- 抗体名：
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Monoclonal Antibody
- 规格：
100ul/50ul
| 规格： | 100ul | 产品价格： | ￥1600.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥960.0 |
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Monoclonal Antibody
产品简介
| 英文名称 | Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid Monoclonal Antibody |
| 靶标 | SARS-CoV/SARS-CoV-2 Nucleocapsid |
| 别名 | 2019-nCoV Nucleocapsid;SARS-CoV-2 Nucleocapsid Protein;SARS-CoV-2 NP;2019-nCoV N;Coronavirus NP |
| 宿主 | Mouse |
| 稀释比例 | WB 1:5000-8000. ELISA 1:5000-50000. |
| Gene ID | 43740575/1489678 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| Swiss Prot | P0DTC9/P59595 |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 蛋白分子量 | 46kDa |
| 免疫原 | Recombinant Protein |
| 亚型 | IgG1 |
| 纯化方法 | Affinity purification |
| 克隆号 | 2C12 |
| 背景资料 | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| 克隆类型 | Monoclonal Antibody |
| 交叉反应 | SARS-CoV SARS-CoV-2 |
| 应用 | WB Elisa |
| 规格 | 50ul 100ul |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图 
Western blot analysis with SARS-CoV/SARS-CoV-2 Nucleocapsid antibody diluted at 1:5000.
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- 作者
- 内容
- 询问日期
文献和实验Establishment and Characterization of Monoclonal Antibodies Against SARS Coronavirus
against severe acute respiratory syndrome coronavirus (SARS-CoV). B cell hybridomas are generated from mice that are hyperimmunized with inactivated SARS-CoV virions. The hybridomas produce monoclonal antibodies that recognize viral component molecules, including
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
4. Remove solution and rinse ovaries with PBS 3 times, then wash 3 times for 10 minutes each 5. It may help antibody penetration to dissect apart the egg chambers 6. Incubate ovaries in PBT for 10 minutes 7. Add monoclonal supernatant
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