Anti-CCAR2 Polyclonal Antibody

Anti-CCAR2 Polyclonal Antibody


产品简介

英文名称	Anti-CCAR2 Polyclonal Antibody
靶标	CCAR2
别名	DBC-1;DBC1;KIAA1967;NET35;p30 DBC;p30DBC
宿主	Rabbit
稀释比例	WB 1:1000-3000. IHC 1:100-300.
Gene ID	57805
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	Q8N163
蛋白分子量	103kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Nucleus
免疫原	Recombinant protein of human CCAR2
亚型	IgG
纯化方法	Affinity purification
背景资料	Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. Mediates ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation. Represses the ligand-dependent transcriptional activation function of ESR2. Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1. Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization. Positively regulates the beta-catenin pathway (canonical Wnt signaling pathway) and is required for MCC-mediated repression of the beta-catenin pathway. Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3. Plays an important role in tumor suppression through p53/TP53 regulation; stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2. Represses the transcriptional activator activity of BRCA1. Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro.
Public Immunogen Range	1-350aa
克隆类型	Polyclonal Antibody
交叉反应	Human
应用	WB IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图

Western blot analysis with CCAR2 antibody diluted at 1:2000;Lane:HEK-293,HeLa,COS7,NIH3T3

Immunohistochemistry of paraffin-embedded Human cervical cancer with CCAR2 antibody diluted at 1:200

Immunohistochemistry of paraffin-embedded Human endometrial cancer with CCAR2 antibody diluted at 1:200

Immunohistochemistry of paraffin-embedded Human liver cancer cancer with CCAR2 antibody diluted at 1:200