Anti-WIPI1 Polyclonal Antibody

Anti-WIPI1 Polyclonal Antibody



产品简介

英文名称	Anti-WIPI1 Polyclonal Antibody
靶标	WIPI1
别名	ATG18;ATG18A;WIPI49
宿主	Rabbit
稀释比例	WB 1:1000-3000. IHC 1:50-200.
Gene ID	55062
保存	Store at -20°C. Avoid freeze / thaw cycles.
Swiss Prot	Q5MNZ9
蛋白分子量	48kDa
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
亚细胞定位	Cytoplasm
免疫原	Recombinant protein of human WIPI1
亚型	IgG
纯化方法	Affinity purification
背景资料	Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway.
Public Immunogen Range	1-350aa
克隆类型	Polyclonal Antibody
交叉反应	Human Mouse
应用	WB IHC
规格	50ul 100ul

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图

Western blot analysis with WIPI1 antibody diluted at 1:2000;Lane:Mouse eye

Immunohistochemistry of paraffin-embedded Human colorectal cancer with WIPI1 antibody diluted at 1:100

Immunohistochemistry of paraffin-embedded Human endometrial cancer with WIPI1 antibody diluted at 1:100