- ￥960
- Solarbio
- 中国 北京
- K110231P-50ul
- 2025年07月12日
- WB
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide of human EIF3E
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB
- 靶点：
EIF3E
- 规格：
50ul
产品简介
| 英文名称 | Anti-EIF3E Polyclonal Antibody |
| 靶标 | FARP2 |
| 别名 | eIF-3 p48; eIF3e; EIF3E_HUMAN; EIF3S6; eIFe; Eukaryotic translation initiation factor 3 subunit 6; Eukaryotic translation initiation factor 3 subunit E; eukaryotic translation initiation factor 3, subunit 6 (48kD); INT6; mammary tumor-associated protein INT6; murine mammary tumor integration site 6 (oncogene homolog); Viral integration site protein INT-6 homolog; |
| 宿主 | Rabbit |
| 稀释比例 | WB 1:1000-3000. |
| Gene ID | 3646 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| Swiss Prot | P60228 |
| 蛋白分子量 | 52kDa |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 亚细胞定位 | Cytoplasm |
| 免疫原 | A synthetic peptide of human EIF3E |
| 亚型 | IgG |
| 纯化方法 | Affinity purification |
| 背景资料 | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway (PubMed:17468741). May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (PubMed:17310990, PubMed:17324924). |
| Public Immunogen Range | 380-430/445aa |
| 克隆类型 | Polyclonal Antibody |
| 交叉反应 | Human Rat Mouse |
| 应用 | WB |
| 规格 | 50ul 100ul |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
实验图
Western blot analysis with EIF3E antibody diluted at 1:2000;Lane:Mouse pancreas
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
a simple ELISA method to detect human anti-antibody response.
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