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文献和实验E.Z.N.A.TM Fast Protocol For FFPE Tissue
elution in the following step. 14. Place the column into a sterile 1.5 ml microcentrifuge tube and add 15-50 ul of DEPC Tteated Water. Allow tubes to sit for 3 min at room temperature. 15. To elute RNA from the column, centrifuge at 10,000 x g
E. Z. N.A.TM X-press Plasmid Protocol
at maximum speed (13,000 x g) for 1 minute to dry the column. 14. Transfer the X-press spin column into a new 1.5 ml centrifuge tube. Add 50 uL Elution Buffer (10mM Tris Hcl pH 8.5) directly onto the center of the membrane. 15. Centrifuge
E.Z.N.A.TM Plant DNA Maxi Protocol For Dry Specimens
4 ml of sterile deionized water pre-heated to 65°C and 50ul RNase A (Supplied). Vortex to resuspend the pellet.. Incubate at 65°C for 10 minutes. It may be necessary to remove un-dissolved material by centrifuging at 10,000 x g for 5 minutes. TIP
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