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- 005001
- Invitrogen-CABU
- 2025年12月24日
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文献和实验E L I S A w i t h p l a t e l e t s
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
bath of tap water. 8. Wash sections in TBS* buffer (pH 7.6) for 1 x 5 minutes. 9. Place sections in diluted normal serum (or RTU Normal Horse Serum) for 10 minutes. 10. Incubate sections with primary antibody. 11. Wash in TBS buffer for 2 x
3. Place sections in 0.5% hydrogen peroxide/methanol for 10 minutes (or use other appropriate endogenous peroxidase blocking procedure). Wash sections in tap water. 4. Heat 1500ml of the recommended unmasking solution (0.01M citrate buffer, pH
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