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- CAU24558
- 2025年07月14日
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文献和实验of interest available; 2) formation of the antibody-antigen complex by adding specific antibody; 3) separation and detection of the immune complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Both polyclonal and monoclonal
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
11 Detection of TGF Type II Receptor Hotspot Mutations: The BAT-RII Assay
anchor and intracellular kinase domain. This RII mutation also occurs in gastric and endometrial tumors (4 ) and may be a hot spot in other tumor types with microsatellite instability as well.
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