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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Alanine aminotransferase 2 (GPT2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Alanine aminotransferase 2 (GPT2)品名简称:
Recombinant Human GPT2 protein货号:
CSB-EP823460HU规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
GPT2Uniprot No.:
Q8TD30别名:
(ALT2)(Glutamate pyruvate transaminase 2)(GPT 2)(Glutamic--alanine transaminase 2)(Glutamic--pyruvic transaminase 2)种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
65.3 kDa表达区域:
1-523aa氨基酸序列:
MQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVLKAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.功能2:
/文献:
"cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase." Yang R.-Z., Blaileanu G., Hansen B.C., Shuldiner A.R., Gong D.-W. Genomics 79:445-450(2002)研究领域:
Metabolism请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验Alanine Transaminase Activity Assay
within two months. In the assay (optional), add 5-10 µl positive control and adjust the final volume to 20 µl/well with Alanine Transaminase Assay Buffer. 2. Standard Curve Preparation: 1) Colorimetric assay: Dilute the Pyruvate
【基本原理】 转氨基作用广泛地存在于机体各组织器官中,是体内氨基酸代谢的重要途径。氨基酸反应时均由专一的转氨酶催化,此酶催化氨基酸的α-氨基转移到另一α-酮基酸上。各种转氨酶的活性不同,其中肝脏的谷丙转氨酶(Glutamic Pyruvic Transaminase ,GPT)活性较高,它催化如下反应: α—酮戊二酸 + 丙氨酸 谷氨酸 + 丙酮酸 本实验以丙氨酸和α-酮戊二酸
基作用最重要的氨基受体是α酮戊二酸,产生谷氨酸作为新生成氨基酸: 进一步将谷氨酸中的氨基转给草酰乙酸,生成α酮戊二酸和天冬氨酸: 或转给丙酮酸。生成α酮戊二酸和丙氨酸,通过第二次转氨反应,再生出α酮戊二酸。 因而体内有较强的谷草转氨酸(glutamic pyruvic transaminase,GPT)和谷丙转氨酸(glutamic oxaloacetic trans aminase,GOT)活性。 转氨基作用是可逆的,该反应中
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