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人GPT2重组蛋白(ALT2)(Glutamate pyru

vate transaminase 2)(GPT 2)(Glutamic--alanine transaminase 2)(Glutamic--pyruvic transaminase 2)Human Alanine aminotransferase 2(GPT2)蛋白
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  • ¥1536
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  • CN
  • CSB-EP823460HU
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Human Alanine aminotransferase 2 (GPT2)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Human Alanine aminotransferase 2 (GPT2)

    品名简称:

    Recombinant Human GPT2 protein

    货号:

    CSB-EP823460HU

    规格:

    20μg

    价格:

    1536

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    GPT2

    Uniprot No.:

    Q8TD30

    别名:

    (ALT2)(Glutamate pyruvate transaminase 2)(GPT 2)(Glutamic--alanine transaminase 2)(Glutamic--pyruvic transaminase 2)

    种属:

    Homo sapiens (Human)

    蛋白长度:

    Full Length

    来源:

    E.coli

    分子量:

    65.3 kDa

    表达区域:

    1-523aa

    氨基酸序列:

    MQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVLKAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVMIPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRKCIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA

    蛋白标签:

    N-terminal 10xHis-tagged and C-terminal Myc-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    13-23 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.

    功能2:

    /

    文献:

    "cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase." Yang R.-Z., Blaileanu G., Hansen B.C., Shuldiner A.R., Gong D.-W. Genomics 79:445-450(2002)

    研究领域:

    Metabolism

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    相关实验
    • Alanine Transaminase Activity Assay

      within two months. In the assay (optional), add 5-10 µl positive control and adjust the final volume to 20 µl/well with Alanine Transaminase Assay Buffer. 2.      Standard Curve Preparation: 1)   Colorimetric assay: Dilute the Pyruvate

    • 用纸层析法鉴定转氨酶的转氨基作用

      【基本原理】 转氨基作用广泛地存在于机体各组织器官中,是体内氨基酸代谢的重要途径。氨基酸反应时均由专一的转氨酶催化,此酶催化氨基酸的α-氨基转移到另一α-酮基酸上。各种转氨酶的活性不同,其中肝脏的谷丙转氨酶(Glutamic Pyruvic Transaminase ,GPT)活性较高,它催化如下反应: α—酮戊二酸 + 丙氨酸 谷氨酸 + 丙酮酸 本实验以丙氨酸和α-酮戊二酸

    • 氨基酸的脱氨基作用

      基作用最重要的氨基受体是α酮戊二酸,产生谷氨酸作为新生成氨基酸: 进一步将谷氨酸中的氨基转给草酰乙酸,生成α酮戊二酸和天冬氨酸: 或转给丙酮酸。生成α酮戊二酸和丙氨酸,通过第二次转氨反应,再生出α酮戊二酸。 因而体内有较强的谷草转氨酸(glutamic pyruvic transaminase,GPT)和谷丙转氨酸(glutamic oxaloacetic trans aminase,GOT)活性。 转氨基作用是可逆的,该反应中

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    文献支持
    人GPT2重组蛋白(ALT2)(Glutamate pyruvate transaminase 2)(GPT 2)(Glutamic--alanine transaminase 2)(Glutamic--pyruvic transaminase 2)Human Alanine aminotransferase 2(GPT2)蛋白
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