Product AWS0005 has been successfully used in Immunofluorescence (IF) applications at a dilution from 1:100-1:1,000.
In order to increase specificity of the antibody and eliminatebackground staining, Product AWS0005 has been purified by cross-adsorption or pre-adsorption against mouse IgG,mouse serum and human serum. This secondary antibody was conjugated with Alex Fluor 488 dye. Firstly, Alex Fluor 488 dye is well-known as it is a bright, green-fluorescentdye with excitation ideally suited to the 488 nm laser line. Additionally,to obtain stable signal generation in imaging and flow cytometry,Alexa Fluor 488 dye is pH-insensitive over a wide molar range.
Before use please centrifuge solution briefly in a microcentrifuge.