Product AWS0002 has been successfully used in Western blot, IHC and IP applications.
Product AWS0002 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species and with SuperBlock Blocking Buffers.
Store product at -20℃ until opened.
Product Images For Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Rabbit IgG (H+L) Secondary Antibody (AWS0002) in WB Western blot analysis was performed on whole cell extracts (30 µg lysate) of mouse brain tissue (Lane 1, 2, 3, and 4). The blots were probed with Anti-GAPDH Rabbit Polyclonal Antibody (Product 10494-1-AP, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product AWS0002) at dilutions 1:5,000 (Fig. 1, Line 1 and 2)and 1:10,000 (Fig. 1, Line 3 and 4). A 36 kDa band corresponding to GAPDH was observed. Known quantity of protein samples were electrophoresed using 11 % Bis-Tris geland Pre-Stained Protein Standard (Thermo Fisher Scientific, Product 26616). Resolved proteins were then transferred onto a nitrocellulose membrane with Dry Blotting System. The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Advansta SuperECL plus Western Blotting Substrate (Product k-120450). 1 2 3 4