///蛋白Recombinant Abrus precato

纯度：

Greater than 90% as determined by SDS-PAGE.

基因名：

/

Uniprot No.：

P28590

别名：

(rRNA N-glycosidase)

种属：

Abrus precatorius (Indian licorice) (Glycine abrus)

蛋白长度：

Partial

来源：

E.coli

分子量：

35.3 kDa

表达区域：

35-285aa

氨基酸序列：

QDQVIKFTTEGATSQSYKQFIEALRQRLTGGLIHDIPVLPDPTTVEERNRYITVELSNSERESIEVGIDVTNAYVVAYRAGSQSYFLRDAPASASTYLFPGTQRYSLRFDGSYGDLERWAHQTREEISLGLQALTHAISFLRSGASNDEEKARTLIVIIQMASEAARYRYISNRVGVSIRTGTAFQPDPAMLSLENNWDNLSGGVQQSVQDTFPNNVILSSINRQPVVVDSLSHPTVAVLALMLFVCN_P_PN

蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

The A chain is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits by removing adenine from position 4,324 of 28S rRNA. Abrin-a is more toxic than ricin. ; The B chain is a galactose-specific lectin that facilitates the binding of abrin to the cell membrane that precedes endocytosis.

内毒素：

Not test.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

/

Product types:

In Stock Protein

Biological_Activity:

/

Research Areas:

Others

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

"Preproabrin: genomic cloning, characterisation and the expression of the A-chain in Escherichia coli." Wood K.A., Lord J.M., Wawrzynczak E.J., Piatak M. Eur. J. Biochem. 198:723-732(1991)

Function:

/