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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Abrus precatorius Abrin-c, partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Abrus precatorius Abrin-c, partial品名简称:
Recombinant Abrus precatorius Abrin-c protein, partial货号:
CSB-EP340994AAC规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
P28590别名:
(rRNA N-glycosidase)种属:
Abrus precatorius (Indian licorice) (Glycine abrus)蛋白长度:
Partial来源:
E.coli分子量:
35.3 kDa表达区域:
35-285aa氨基酸序列:
QDQVIKFTTEGATSQSYKQFIEALRQRLTGGLIHDIPVLPDPTTVEERNRYITVELSNSERESIEVGIDVTNAYVVAYRAGSQSYFLRDAPASASTYLFPGTQRYSLRFDGSYGDLERWAHQTREEISLGLQALTHAISFLRSGASNDEEKARTLIVIIQMASEAARYRYISNRVGVSIRTGTAFQPDPAMLSLENNWDNLSGGVQQSVQDTFPNNVILSSINRQPVVVDSLSHPTVAVLALMLFVCNPPN蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
The A chain is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits by removing adenine from position 4,324 of 28S rRNA. Abrin-a is more toxic than ricin. ; The B chain is a galactose-specific lectin that facilitates the binding of abrin to the cell membrane that precedes endocytosis.功能2:
/文献:
"Preproabrin: genomic cloning, characterisation and the expression of the A-chain in Escherichia coli." Wood K.A., Lord J.M., Wawrzynczak E.J., Piatak M. Eur. J. Biochem. 198:723-732(1991)研究领域:
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文献和实验578475733 因最近要做蛋白激酶C在糖尿病方面的实验,求助各位高手有没有蛋白激酶C信号通路的图片,最好带注解,十分感谢。 石依信念 我也想要~~~~ 黑胡桃 [img]data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAskAAAGdCAYAAADgwu6RAAAgAElEQVR4Aey9B7xdxZXmu04
质芯片检测。此外,肽芯片或基于质谱的方法[ 20,21] 也可以用于这方面。 我们在此介绍基于蛋白质芯片技术的蛋白磷酸化筛选方法和高通量确定蛋白激酶底物的方法。我们已成功地用这种筛选工具鉴定大麦酪蛋白激酶 2α ( CK 2α) 和不同的拟南芥丝裂原活化蛋白(MAP) 激酶 [23] 的新靶标。我们这个方法使用本书第 28 章详细描述的植物蛋白质芯片(见第 28 章)。在放射性【 γ33 磷】三磷酸腺苷存在的条件下,用可溶和具有活性的激酶孵育芯片。通过磷屏成像仪或 X 射线胶片检测到的放射性信号,对可能
-His-Pro-Gln-Phe-Glu-Lys) 可放在N- 或 C-terminus 不影响蛋白折叠和功能,无需移除 分子量 1058Da Twin-Strep-tag® (也有叫做双Strep-tag®II) 28 aa 标签(WSHPQFEK-GGGSGGGSGG-SA-WSHPQFEK) 两段Strep-tag®II标签序列 较Strep-tag®II对于Strep-Tactin®有更高亲和力 分子量 2887Da 二者均为小标签,不会对蛋白质的结构和功能活性产生影响,因此通常不需要在纯化
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