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文献和实验T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Characterization of Whole Body Cholesterol Fluxes in the Mouse
in this pathway. This article describes in detail a variety of experimental approaches to measure cholesterol fluxes in the hepatobiliary system as well as in the intestinal pathway. Curr. Protoc. Mouse Biol. 1:413?427 © 2011 by John Wiley & Sons
(3 ). PKA is composed of two catalytic (C) and two regulatory (R) subunits and has type I and type II isozymes, with different R subunits, termed RI and RII, interacting with an identical C subunit (1 ). Thus far, four isoforms of R subunits, RIα, RIβ, RIIα
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