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文献和实验(3 ). PKA is composed of two catalytic (C) and two regulatory (R) subunits and has type I and type II isozymes, with different R subunits, termed RI and RII, interacting with an identical C subunit (1 ). Thus far, four isoforms of R subunits, RIα, RIβ, RIIα
Techniques to Study FcRI Signaling
Mast cells are the crucial effector cells for allergic reactions. They are activated through the aggregation of the high-affinity immunoglobulin E (IgE) receptor (FcεRI) with allergen and allergen-specific IgE. Tyrosine phosphorylation of Fcε
Mast Cell Histamine and Cytokine Assays
Mast cells are crucial to the development of chronic allergic inflammation and are likely to play a critical role in host defense. In this chapter methodology for histamine and cytokine assays is provided. Crosslinkage of IgE receptor I (Fcε
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