RORγt inhibitor 1,1561770-72-6

【求助】盼回复miRNA inhibitor相关

lyc_urology 本人，研一，要转染miRNA inhibitor,请教前辈: 1、转染细胞一般inhibitor多少浓度？50nM?25nM? 2、而且本人转然后，需要做MTT，WESTERN等实验，这需要考虑转染效率吗？怎么来判断转染效率呢? 3、顺便问一下，抑制miRNA，就买个inhibitor和阴性对照就行了吧？还需要用阳性对照吗？ 4、转然后分别多久做MTT、Western、克隆形成实验以及流失细胞仪测

The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes: Transcriptional and Posttranscriptional Regulation, Signal

(Table 1 ). The enzymatic activity of MMP family members is controlled by a group of inhibitor proteins known as the Tissue Inhibitors of Metalloproteinases (TIMPs), which consist of four family members (Table 2 ). Whereas all four TIMPs can inhibit

我做的RT-PCR，没有结果，上火啊

录： MgCl2 4ul 10×RNA PCR Buffer 2ul RNase Free dH2O 8.5ul dNTP Mixture(各10mM) 2ul RNase Inhibitor 0.5ul AMV Reverse Transcriptase 1ul Oligo dT-Adaptor Primer 1ul 样品RNA 1ul 30℃ 10min 50℃ 30min 99℃ 5min 5℃ 5min PCR： MgCl2 1.2ul 10×LA RNA PCR