Human BMP-2(人BMP-2) 重组人骨形态发生蛋白

Product Information

 

Product Name	Cat#	Size	Price（¥）
Recombinant Human Bone Morphogenetic Protein 2 (Human BMP-2)	92051ES10	10 μg	1855.00
	92051ES60	100 μg	10215.00
	92051ES76	500 μg	26545.00

 

Product Description

 

Bone morphogenetic protein 2 (BMP-2), is a member of the BMP subgroup, some of which belong to the TGF-beta superfamily (BMP2-7). It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. Normal bone formation is a prolonged process that is carefully regulated and involves sequential expression of growth regulatory factors by osteoblasts as they proliferate and ultimately differentiate. Since this orderly sequence of gene expression by osteoblasts suggests a cascade effect, and BMP-2 is capable of initiating and maintaining this effect. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility.

 

Product Properties

 

Synonyms	BDA2, BMP2, BMP-2, BMP-2A, SSFSC
Accession	P12643
GeneID	650
Source	E.coli-derived human BMP-2, Gln283-Arg396, with an N-terminal Met.
Molecular Weight	Approximately 26.0 kDa.
AA Sequence	MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
Tag	None
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	>95 % by SDS-PAGE and HPLC analyses.
Biological Activity	The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/mL, corresponding to a specific activity of > 5.0 × 103 IU/mg. Fully biologically active when compared to standard.
Endotoxin	< 1.0 EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM sodium citrate, pH 3.5.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in PBS or cell culture media.

 

Shipping and Storage

 

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

 

Avoid repeated freeze-thaw cycles.

For your safety and health, please wear lab coats and disposable gloves for operation.

For research use only.

 

HB220210