Strep-Tactin® Sepharose® 50 % suspension can be used for the purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. The resin is particularly suited for the purification of proteins with multiple tags, such as multimers, that cannot be eluted from Strep-Tactin®XT resins because of the increased affinity. It can be used for the purification of large proteins, due to the low percentage of agarose. Strep-Tactin® irreversibly binds biotin, so if free biotin is expected use BioLock. The suspension format allows the packing of gravity flow columns with custom resin volumes. For a 1 ml resin bed volume, use 2 ml 50 % suspension.
Strep-Tactin® Sepharose® is not compatible with HPLC/FPLC due to a low pressure stability.
For information on our other resins, please check the resin specifications.
Specifications
Bead Size:
45 - 165 µm
Biotin Binding Capacity:
> 300 nmol/ml resin
Exclusion Limit:
3 x 10^7
Form:
50% suspension
Possible Application:
Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins