- ￥535
- 翌圣生物（Yeasen）
- 90621ES08
- 上海
- 2025年10月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
有效期1年
- 保存条件：
-20℃至-80℃保存
- 供应商：
翌圣生物科技(上海)股份有限公司
- 库存：
大量
- 英文名：
Recombinant Mouse Tumor Necrosis Factor-alpha/TNFSF2 (Mouse TNF-α/TNFSF2)
- 规格：
5μg
| Product Name |
Cat# |
Size |
Price（¥） |
| Recombinant Mouse Tumor Necrosis Factor-alpha/TNFSF2 (Mouse TNF-α/TNFSF2) |
90621ES08 |
5 μg |
815.00 |
| 90621ES60 |
100 μg |
7865.00 |
|
| 90621ES76 |
500 μg |
20425.00 |
Product Description
Tumor necrosis factor alpha (TNF-α), also known as cachectin, is a cachectin, is a polypeptide cytokine produced by monocytes and macrophages. TNFα receptor signaling can simultaneously activate caspase 8, the transcription factor, NF-κB and the kinase, JNK. Human TNF-α shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. Inflammation is associated with insulin resistance, and TNF-α and IL-6 may affect glucose uptake. A wide variety of evidence has pointed to a critical role of TNF-α in tumour proliferation, migration, invasion and angiogenesis.
Product Properties
| Synonyms |
Tumor Necrosis Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing factor, DIF |
| Accession |
|
| GeneID |
|
| Source |
E.coli-derived mouse TNF-α/TNFSF2 protein, Leu80-Leu235, with an N-terminal Met. |
| Molecular Weight |
Approximately 17.4 kDa. |
| AA Sequence |
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
| Tag |
None |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity |
> 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity |
The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg in the presence of actinomycin D. Fully biologically active when compared to standard. |
| Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only!
HB220210
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文献和实验CIRBP 期刊：Journal of Autoimmunity 影响因子：14.511 研究领域：炎症性肠病 主要应用：构建Ad-m-CIRBP-shRNA和Ad-NC，通过腹腔注射至Ets-1 Tg小鼠体内，每只小鼠的剂量为2×1010 PFU，2天后采用TNBS诱导结肠炎模型。研究结果表明，下调 CIRBP表达可抑制Th1细胞分化和TNF-α的产生，以减轻TNBS诱导 Est-1 Tg小鼠结肠 Figure S5D 敲低CIRBP可下调CD4+T细胞部分相关细胞因子的表达
基本原理 TNF的生物学活性之一是能直接杀伤肿瘤细胞。TNF与相应受体结合后向细胞内移，被靶细胞溶酶体摄取导致溶酶体稳定性降低，各种酶外泄，引起细胞溶解。肿瘤细胞株对TNF-α的敏感性有很大的差异，用放线菌素D、丝裂酶素C、放线菌酮等处理肿瘤细胞，可明显增强TNF-α杀伤肿瘤细胞活性。 材料和试剂 1，完全培养基（1）10%FCS-DMEM培养液（V/V）。 2，完全培养基（2）3%FCS-DMEM培养液（V/V）0.5-1μg/ml放线菌素-D。 3，0.05%结晶
笔者前一段时间忙于为新文章制作配图，多处搜集配图优雅美观的优质论文。最后终于找到一篇发表在 Science Advances 的文章——通过高通量测序分析揭示了小 RNA 在小鼠卵母细胞和早期胚胎中的调控作用。文章中除了复杂的 RNA Seq、Chip Seq 分析，图表的搭配十分美观。虽说我们是崇高的科（ke）学 (yan) 家 (gou)，但关乎我们毕业大计的 SCI 也得要「高颜值」。以常见的热图（heatmap）为例，在分子生物学涉及高通量以及芯片的文章中，尤其是 RNA-seq 相关
