- 询价
- Biotium
- 美国
- 22030-50mL
- 2025年07月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
室温
- 保质期：
12个月
- 英文名：
AntiFix™ Universal Antigen Retreival Buffer, 10X, 50 mL 22030-50mL
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
AntiFix™ Universal Antigen Retrieval Buffer is designed for heat-induced epitope retrieval (HIER) of formalin-fixed, paraffin embedded (FFPE) tissues. The buffer uses a proprietary reagent that catalyzes the reversal of formaldehyde cross-links in FFPE tissue to restore antigen reactivity.
Features:
- Excellent antigen retrieval for a wide variety of targets at neutral pH
- Simplifies HIER optimization
- Validated with fluorescence staining using tyramide amplification
- Non-toxic and non-flammable
Fixation of tissue with formalin (formaldehyde) preserves tissue morphology, but the cross-linking of proteins by formaldehyde can modify antibody binding sites or epitopes so they are no longer detectable by immunohistochemistry. Traditionally, epitope reactivity is recovered by using protease treatment, or by heating slides in either low pH citrate buffer or high pH Tris buffer. The optimal method for unmasking varies for different epitopes, requiring multiple buffers to be used for different targets.
AntiFix™ Universal Antigen Retrieval Buffer has been formulated to provide excellent heat unmasking for a wide variety of targets at neutral pH, reducing the need for buffer optimization. It has been validated in immunofluorescence staining of FFPE tissue with our next-generation fluorescent CF® dyes using HRP-mediated tyramide signal amplification.
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文献和实验after use), combine to make 5 ml of 10X reaction buffers, put 1 ml in to each of 5 microfuge tubes (MFTs) and store the current one at -20℃ and the rest at -70℃.A: 1 M Tris-HCl 50 ml: 4.88 g Tris-HCl + 2.3 g Tris-Base (pH 7.9 at 25℃)B: 1 M Tris-HCl 50 ml
. For these challenging antigen-down assays CANDOR has developed PlateBlock™, which was optimized to prevent the exchange reactions in the most comprehensive way by forming a very resilient blocking layer on plastic surfaces (Fig. 2). In combination with LowCross
A general protocol for staining cell for cytometry analysis
(Cat. No. 66121A) : 0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1X prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1X PBS buffer. Store at RT. Recommended for use in parallel with Annexin
