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- 文献和实验
- 技术资料
- 保存条件：
保存在-20℃，1年。
- 保质期：
保存在-20℃，1年。
- 英文名：
Lysozyme
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- CAS号：
12650-88-3
- 规格：
3ml
产品简介
| 别名 | 胞壁质酶；Globulin G1；Muramidase |
| 英文名称 | Lysozyme |
| CAS | 12650-88-3 |
| 外观（性状） | 液体 |
| 单位 | 瓶 |
| 规格 | 3ml 6ml |
产品说明:
溶菌酶主要用于酶分解细菌的细胞壁；用于制备原生质球。溶菌酶对革兰氏阳性菌、好气性孢子形成菌、枯草杆菌、耐辐射微球菌均有良好的分解作用,对大肠杆菌、普通变球菌和副溶血性弧菌等革兰氏阴性菌也有一定程度的溶解作用。溶菌酶具有分解细菌细胞壁中肽聚糖的特殊作用。
常见问题
Q：请问可以用来做WB吗?
A：您好，溶菌酶可用于提取革兰氏阳性菌和革兰氏阴性菌蛋白提取实验中，水解革兰氏阳性菌和革兰氏阴性菌的细胞壁。
Q：可以用 高压过的ddH20进行稀释吗?
A：您好，本品可溶于水，应根据需要选择合适的溶剂。
Q：溶菌酶液体是直接萃取出来的还是用溶剂稀释的呢?
A：您好，本品是用溶剂稀释得到的。
Q：使用时如何溶解?
A：您好，该产品为液体形式，浓度为100mg/ml，若需要稀释可用灭菌水。
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/ LNaCI (2)50 mmol / L 苯甲基磺酰氟(PMSF ). (3)10 mg / mL 溶菌酶. (4)脱氧胆酸. (5)1 mg / mL DNase I. 2 )超声破碎法 ( 1 ) TE 缓冲液. ( 2 ) 2×SDS -PAGE 凝胶电泳加样缓冲液： 100 mmol / L Tris-HCI ( pH 8 . 0 ) 100 mmol / L DTT 4 %SDS 0.2 % 溴酚
How to Make Simple Solutions and Dilutions
= 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V1 = (V2 x C2) / C1 V1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and after cancelling the units, V1 = 0.05 ml, or 50 ul
, resuspend in 2 ml 1 x LiAc/ 0.5 x TE incubate at RT for 10 min combine in tube: 100 µl cells, 10 µl salmon sperm DNA (10 mg/ml), up to 15 µl DNA to be transformed add 700 µl 1 x LiAc / 1 x TE / 40 % PEG mix incubate for 30 min
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