干扰素γ(IFNg)活性蛋白

干扰素γ(IFNg)活性蛋白

Active Interferon Gamma (IFNg)

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

 

• 物种Homo sapiens (Human，人)

• 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
  
  

• 性状冻干粉

• 纯度> 95%

• 等电点9.7

• 应用Cell culture; Activity Assays.

• 规格10µg50µg 200µg 1mg 5mg

• 活性实验

   

  

  IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons.The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. As reported, IFNγ is an important activator of human monocytic THP1 cells. Therefore,THP-1 cells were incubated in RPMI 1640 with various concentration of IFN-γ, then cells were observed by inverted microscope everyday.After stimulated with IFN-γ(5ng/ml) for 5 days, morphological changes occured in THP1 cells which displayed the shape of fusiform or polygon and were more likely to adhere.<br/>Effect of IFN-γ on THP1 cells is shown in Figure 1.

  
  

   

  

  用法

  Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

  储存

  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

  稳定性

  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。