优利科（上海）生命科学有限公司
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上海 奉贤区
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重组蛋白注意事项
79 人阅读发布时间：2022-02-16 14:00
REAGENTS PROVIDED
All reagents provided are stored at 2-8° C. Refer to the expiration date on the label.
1. MICROTITER PLATE 96 wells
2. Biotinylated anti-IgE 6.0 mL 1 tube
3. STANDARD(320ng/ml) 0.5ml 1 tube
4. STREPTAVIDIN-HRP 6.0 mL 1 tube
5. STANDARD DILUENT 1.5ml 1 tube
6. Chromogen Solution A 6.0 mL 1 vial
7. Chromogen Solution B 6.0 mL 1 vial
8. STOP SOLUTION 6.0 mL 1 vial
9. WASH SOLUTION x30 20 mL 1 vial
10. Instruction 1
SAMPLE COLLECTION AND STORAGE
Serum- Use a serum separator tube(SST) and allow samples to clot for 30minutes before centrifugation for 15minutes at approximately 1000 x g.Remove serum and assay immediately or aliquot and store samples at -20 °C or -80°C.
Plasma - Collect plasma using EDTA or heparin as an anticoagulant.Centrifuge samples for 15 minutes at 1000 x g at 2-8°C within 30minutes of collection.Store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
Cell culture fluid and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
NOTE: Serum, plasma, and cell culture fluid samples to be used within 7 days may be stored at 2-8°C, otherwise samples must stored at -20°C(≤2months) or -80°C(≤6months) to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles .When performing the assay slowly bring samples to room temperature.
DO NOT USE HEAT-TREATED SPECIMENS.
MATERIALS REQUIRED BUT NOT SUPPLIED
1. Microplate reader capable of measuring absorbance at 450 nm.
2. Precision pipettes to deliver 2 ml to 1 ml volumes.
3. Adjustable 10ml -100ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Calibrated adjustable precision pipettes, preferably with disposable plastic tips. (A manifold multi-channel pipette is desirable for large assays.)
6. Absorbent paper.
7. 37°C incubator.
8. Distilled or deionized water.
9. Data analysis and graphing software.
10. Tubes to prepare standard or sample dilutions.
PRECAUTIONS
1. Do not substitute reagents from one kit lot to another. Standard, conjugate and microtiter plates are matched for optimal performance. Use only the reagents supplied by manufacturer.
2. Allow kit reagents and materials to reach room temperature (20-25°C) before use. Do not use water baths to thaw samples or reagents.
3. Do not use kit components beyond their expiration date.
4. Use only deionized or distilled water to dilute reagents.
5. Do not remove microtiter plate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
6. Use fresh disposable pipette tips for each transfer to avoid contamination.
7. Do not mix acid and sodium hypochlorite solutions.
8. Serum and plasma should be handled as potentially hazardous and capable of transmitting disease. Disposable gloves must be worn during the assay procedure, since no known test method can offer complete assurance that products derived from human blood will not transmit infectious agents. Therefore, all blood derivatives should be considered potentially infectious and good laboratory practices.
FOR LABORATORY RESEARCH USE ONLY.
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
All reagents provided are stored at 2-8° C. Refer to the expiration date on the label.
1. MICROTITER PLATE 96 wells
2. Biotinylated anti-IgE 6.0 mL 1 tube
3. STANDARD(320ng/ml) 0.5ml 1 tube
4. STREPTAVIDIN-HRP 6.0 mL 1 tube
5. STANDARD DILUENT 1.5ml 1 tube
6. Chromogen Solution A 6.0 mL 1 vial
7. Chromogen Solution B 6.0 mL 1 vial
8. STOP SOLUTION 6.0 mL 1 vial
9. WASH SOLUTION x30 20 mL 1 vial
10. Instruction 1
SAMPLE COLLECTION AND STORAGE
Serum- Use a serum separator tube(SST) and allow samples to clot for 30minutes before centrifugation for 15minutes at approximately 1000 x g.Remove serum and assay immediately or aliquot and store samples at -20 °C or -80°C.
Plasma - Collect plasma using EDTA or heparin as an anticoagulant.Centrifuge samples for 15 minutes at 1000 x g at 2-8°C within 30minutes of collection.Store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
Cell culture fluid and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
NOTE: Serum, plasma, and cell culture fluid samples to be used within 7 days may be stored at 2-8°C, otherwise samples must stored at -20°C(≤2months) or -80°C(≤6months) to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles .When performing the assay slowly bring samples to room temperature.
DO NOT USE HEAT-TREATED SPECIMENS.
MATERIALS REQUIRED BUT NOT SUPPLIED
1. Microplate reader capable of measuring absorbance at 450 nm.
2. Precision pipettes to deliver 2 ml to 1 ml volumes.
3. Adjustable 10ml -100ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Calibrated adjustable precision pipettes, preferably with disposable plastic tips. (A manifold multi-channel pipette is desirable for large assays.)
6. Absorbent paper.
7. 37°C incubator.
8. Distilled or deionized water.
9. Data analysis and graphing software.
10. Tubes to prepare standard or sample dilutions.
PRECAUTIONS
1. Do not substitute reagents from one kit lot to another. Standard, conjugate and microtiter plates are matched for optimal performance. Use only the reagents supplied by manufacturer.
2. Allow kit reagents and materials to reach room temperature (20-25°C) before use. Do not use water baths to thaw samples or reagents.
3. Do not use kit components beyond their expiration date.
4. Use only deionized or distilled water to dilute reagents.
5. Do not remove microtiter plate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
6. Use fresh disposable pipette tips for each transfer to avoid contamination.
7. Do not mix acid and sodium hypochlorite solutions.
8. Serum and plasma should be handled as potentially hazardous and capable of transmitting disease. Disposable gloves must be worn during the assay procedure, since no known test method can offer complete assurance that products derived from human blood will not transmit infectious agents. Therefore, all blood derivatives should be considered potentially infectious and good laboratory practices.
FOR LABORATORY RESEARCH USE ONLY.
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
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