神经生长因子(NGF)活性蛋白

神经生长因子(NGF)活性蛋白

Active Nerve Growth Factor (NGF)

NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor

 

• 物种Rattus norvegicus (Rat，大鼠) 

• 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
  
  
  

• 性状冻干粉

• 纯度> 90%

• 等电点9.4

• 应用Cell culture; Activity Assays.

• 规格10µg50µg 200µg 1mg 5mg

  活性实验

   

  

  Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance, proliferation, and survival of certain target neurons. NGF is also involved in pathways besides those regulating the life cycle of neurons. a nerve growth factor (NGF)-dependent TF-1 subclone cell line suitable for determination of bioactivity of recombinant NGF(rhNGF), then TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well with 1% serum standard 1640 contain various concentrations of recombinant rat NGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of TF-1 cells after incubation with TF-1 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant NGF for 72h. The result was shown in Figure 2. It was obvious that NGF significantly increased cell viability of TF-1 cells.
  (A) TF-1 cells cultured in 1640, stimulated with 0.1ng/mL NGF for 72h;
  (B) Unstimulated TF-1 cells cultured in 1640 for 72h.
  Figure. Cell proliferation of TF-1 cells after stimulated with NGF.

  
  

  Figure. Cell proliferation of TF-1 cells after stimulated with NGF.

   

  用法

  Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

  储存

  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

  稳定性

  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。