MDA-MB-435S/MDA-MB-435S/MDA-MB

MDA-MB-435S/MDA-MB-435S细胞系/MDA-MB-435S细胞株/MDA-MB-435S人乳腺导管癌细胞

Cell line name MDA-MB-435S

Synonyms MDA-MB-435s; MDA-MB-435 S; MDA-MB-435-S; MDAMB435S; BrCL15

Accession CVCL_0622

Resource Identification Initiative To cite this cell line use: MDA-MB-435S (RRID:CVCL_0622)

Comments Problematic cell line: Contaminated. Parent cell line (MDA-MB-435) has been shown to be a M14 derivative.

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: KuDOS 95 cell line panel.

Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00565.

Population: Caucasian.

Microsatellite instability: Stable (MSS) (PubMed=12661003).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: miRNA expression profiling.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.

Sequence variations

Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (PubMed=19593635; DepMap=ACH-000884).

Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (PubMed=19593635).

Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (PubMed=19593635).

Mutation; HGNC; 3373; EP300; Simple; p.Leu827Pro (c.2480T>C); Zygosity=Hemizygous (PubMed=10700188).

Mutation; HGNC; 3373; EP300; Simple; p.Glu1013Gly (c.3038A>G); ClinVar=VCV000562024; Zygosity=Hemizygous (PubMed=10700188).

Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (PubMed=16541312; DepMap=ACH-000884).

HLA typing Source: PubMed=26589293

Class I

HLA-A A*24:02,24:02

HLA-B B*15:01,15:01

HLA-C C*03:03,03:03

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0.01

Native American 0

East Asian, North 2.13

East Asian, South 0

South Asian 0

European, North 65.23

European, South 32.63

Disease Amelanotic melanoma (NCIt: C3802)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Parent: CVCL_0417 (MDA-MB-435)

Children:

CVCL_A6LJ (MDA-MB-435S/1) CVCL_5T70 (MDA-MB-435s-mKate2)

Sex of cell Male

Age at sampling 33Y

Category Cancer cell line

STR profile Source(s): ATCC=HTB-129; CCRID; CLS=300277; PubMed=28940260

 

Markers:

Amelogenin X

CSF1PO 11 (ATCC=HTB-129; CCRID; CLS=300277)

11,12 (PubMed=28940260)

D1S1656 12

D2S1338 19,24

D3S1358 14 (ATCC=HTB-129; CLS=300277; PubMed=28940260)

14,20 (CCRID)

D5S818 11,12 (CCRID)

12 (ATCC=HTB-129; CLS=300277; PubMed=28940260)

D6S1043 11

D7S820 8 (PubMed=28940260)

8,10 (ATCC=HTB-129; CCRID; CLS=300277)

D8S1179 13 (ATCC=HTB-129; CCRID; CLS=300277)

13,14 (PubMed=28940260)

D12S391 18,19

D13S317 12 (ATCC=HTB-129; CCRID; PubMed=28940260)

12,13 (CLS=300277)

D16S539 13

D18S51 13 (ATCC=HTB-129; PubMed=28940260)

13,17 (CLS=300277)

D19S433 14 (CCRID)

14,15 (ATCC=HTB-129; CLS=300277; PubMed=28940260)

D21S11 30

FGA 21

Penta D 9,11

Penta E 10,12

TH01 6,7

TPOX 8,11

vWA 16,18

 

PubMed=2007622; DOI=10.1083/jcb.113.1.173; PMCID=PMC2288921

Frixen U.H., Behrens J., Sachs M., Eberle G., Voss B., Warda A., Lochner D., Birchmeier W.

E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells.

J. Cell Biol. 113:173-185(1991)

 

DOI=10.1016/B978-0-12-333530-2.50009-5

Leibovitz A.

Cell lines from human breast.

(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.161-184; Academic Press; New York; USA (1994)

 

PubMed=10700188; DOI=10.1038/73536

Gayther S.A., Batley S.J., Linger L., Bannister A.J., Thorpe K., Chin S.-F., Daigo Y., Russell P., Wilson A., Sowter H.M., Delhanty J.D.A., Ponder B.A.J., Kouzarides T., Caldas C.

Mutations truncating the EP300 acetylase in human cancers.

Nat. Genet. 24:300-303(2000)

 

PubMed=12354931; DOI=10.1136/mp.55.5.294; PMCID=PMC1187258

Ellison G., Klinowska T., Westwood R.F.R., Docter E., French T., Fox J.C.

Further evidence to support the melanocytic origin of MDA-MB-435.

Mol. Pathol. 55:294-299(2002)

 

PubMed=12661003; DOI=10.1002/gcc.10196

Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.F., Schlag P.M., Scherneck S.

Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.

Genes Chromosomes Cancer 37:29-35(2003)

 

PubMed=15677628; DOI=10.1093/carcin/bgi032

Gorringe K.L., Chin S.-F., Pharoah P.D.P., Staines J.M., Oliveira C., Edwards P.A.W., Caldas C.

Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer.

Carcinogenesis 26:923-930(2005)

 

PubMed=16397213; DOI=10.1158/0008-5472.CAN-05-2853

Elstrodt F., Hollestelle A., Nagel J.H.A., Gorin M., Wasielewski M., van den Ouweland A.M.W., Merajver S.D., Ethier S.P., Schutte M.

BRCA1 mutation analysis of 41 human breast cancer cell lines reveals three new deleterious mutants.

Cancer Res. 66:41-45(2006)

 

PubMed=16541312; DOI=10.1007/s10549-006-9186-z

Wasielewski M., Elstrodt F., Klijn J.G.M., Berns E.M.J.J., Schutte M.

Thirteen new p53 gene mutants identified among 41 human breast cancer cell lines.

Breast Cancer Res. Treat. 99:97-101(2006)

 

PubMed=19593635; DOI=10.1007/s10549-009-0460-8

Hollestelle A., Nagel J.H.A., Smid M., Lam S., Elstrodt F., Wasielewski M., Ng S.S., French P.J., Peeters J.K., Rozendaal M.J., Riaz M., Koopman D.G., ten Hagen T.L.M., de Leeuw B.H.C.G.M., Zwarthoff E.C., Teunisse A., van der Spek P.J., Klijn J.G.M., Dinjens W.N.M., Ethier S.P., Clevers H.C., Jochemsen A.G., den Bakker M.A., Foekens J.A., Martens J.W.M., Schutte M.

Distinct gene mutation profiles among luminal-type and basal-type breast cancer cell lines.

Breast Cancer Res. Treat. 121:53-64(2010)