CAL-62/CAL-62/CAL-62/人甲状腺癌细胞

CAL-62/CAL-62细胞系/CAL-62细胞株/CAL-62人甲状腺癌细胞

Cell line name CAL-62

Synonyms Cal-62; CAL 62; Cal 62; CAL62; Centre Antoine Lacassagne-62

Accession CVCL_1112

Resource Identification Initiative To cite this cell line use: CAL-62 (RRID:CVCL_1112)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Population: Caucasian.

Doubling time: ~24 hours (DSMZ=ACC-448).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Thyroid gland; UBERON=UBERON_0002046.

Sequence variations

Mutation; HGNC; 2348; CREBBP; Simple; p.Glu1541Ter (c.4621G>T); Zygosity=Heterozygous (PubMed=30737244).

Mutation; HGNC; 3373; EP300; Simple; p.Asp1485fs (c.4454delA); Zygosity=Homozygous (PubMed=30737244).

Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Arg (c.34G>C); ClinVar=VCV000012579; Zygosity=Homozygous (PubMed=23833040; PubMed=30737244).

Mutation; HGNC; 7773; NF2; Simple; p.Glu215Ter (c.643G>T); Zygosity=Homozygous (PubMed=30737244).

Mutation; HGNC; 11998; TP53; Simple; p.Ala161Asp (c.482C>A); ClinVar=VCV000422295; Zygosity=Homozygous (PubMed=30737244).

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0.55

Native American 0.27

East Asian, North 1.91

East Asian, South 0

South Asian 1.04

European, North 46.22

European, South 50.02

Disease Thyroid gland anaplastic carcinoma (NCIt: C3878)

Anaplastic thyroid carcinoma (ORDO: Orphanet_142)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Sex of cell Female

Age at sampling 70Y

Category Cancer cell line

STR profile Source(s): Cosmic-CLP=906828; DSMZ=ACC-448; PubMed=18713817; PubMed=25365311; PubMed=30737244

 

Markers:

Amelogenin X

CSF1PO 9,12

D2S1338 19,23

D3S1358 16

D5S818 9,12

D7S820 10

D8S1179 13

D13S317 12

D16S539 12,13

D18S51 16

D19S433 14

D21S11 32.2

FGA 19

Penta D 13

Penta E 5,10

TH01 7,9

TPOX 8,9

vWA 16

 

Run an STR similarity search on this cell line

Publications

PubMed=1369551

Gioanni J., Zanghellini E., Mazeau C., Zhang D., Courdi A., Farges M.-F., Lambert J.-C., Duplay H., Schneider M.

Characterization of a human cell line from an anaplastic carcinoma of the thyroid gland.

Bull. Cancer 78:1053-1062(1991)

 

PubMed=18713817; DOI=10.1210/jc.2008-1102; PMCID=PMC2582569

Schweppe R.E., Klopper J.P., Korch C.T., Pugazhenthi U., Benezra M., Knauf J.A., Fagin J.A., Marlow L.A., Copland J.A. 3rd, Smallridge R.C., Haugen B.R.

Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification.

J. Clin. Endocrinol. Metab. 93:4331-4341(2008)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

 

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

 

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027

Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Nature 483:603-607(2012)

 

PubMed=23833040; DOI=10.1210/jc.2013-2383; PMCID=PMC3763971

Landa I., Ganly I., Chan T.A., Mitsutake N., Matsuse M., Ibrahimpasic T., Ghossein R.A., Fagin J.A.

Frequent somatic TERT promoter mutations in thyroid cancer: higher prevalence in advanced forms of the disease.

J. Clin. Endocrinol. Metab. 98:E1562-E1566(2013)

 

PubMed=25365311; DOI=10.1210/jc.2014-2359; PMCID=PMC4318896

Garg M., Okamoto R., Nagata Y., Kanojia D., Venkatesan S., Anand M.T., Braunstein G.D., Said J.W., Doan N.B., Ho Q., Akagi T., Gery S., Liu L.-Z., Tan K.T., Chang W.J., Yang H.H., Ogawa S., Koeffler H.P.

Establishment and characterization of novel human primary and metastatic anaplastic thyroid cancer cell lines and their genomic evolution over a year as a primagraft.

J. Clin. Endocrinol. Metab. 100:725-735(2015)

 

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469

Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., M, Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.

A landscape of pharmacogenomic interactions in cancer.

Cell 166:740-754(2016)

 

PubMed=30737244; DOI=10.1158/1078-0432.CCR-18-2953; PMCID=PMC6522280

Landa I., Pozdeyev N., Korch C.T., Marlow L.A., Smallridge R.C., Copland J.A. 3rd, Henderson Y.C., Lai S.Y., Clayman G.L., Onoda N., Tan A.-C., Garcia-Rendueles M.E.R., Knauf J.A., Haugen B.R., Fagin J.A., Schweppe R.E.

Comprehensive genetic characterization of human thyroid cancer cell lines: a validated panel for preclinical studies.

Clin. Cancer Res. 25:3141-3151(2019)