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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Neuro-2a
- 库存：
1x10^6/瓶/支
- 供应商：
上海酶研
- 肿瘤类型：
详询
- 细胞类型：
脑神经瘤细胞
- ATCC Number：
详询
- 品系：
Neuro-2a
- 组织来源：
脑神经瘤细胞
- 相关疾病：
Neuro-2a
- 物种来源：
哺乳动物
- 免疫类型：
详询
- 细胞形态：
贴壁/悬浮
- 是否是肿瘤细胞：
是
- 器官来源：
脑神经瘤细胞
- 运输方式：
顺丰快递
- 年限：
5年
- 生长状态：
生长良好
Neuro-2a/Neuro-2a细胞系/Neuro-2a细胞株/Neuro-2a脑神经瘤细胞
Cell line name Neuro-2a
Synonyms NEURO-2A; Neuro 2a; Neuro2a; Neuro2A; N-2a; N2a; N2A; Nb2a; NB2a
Accession CVCL_0470
Resource Identification Initiative To cite this cell line use: Neuro-2a (RRID:CVCL_0470)
Comments Characteristics: Capable of differentiating into various types of neurons.
Doubling time: ~70 hours (DSMZ=ACC-148).
Omics: Deep proteome analysis.
Omics: SNP array analysis.
Miscellaneous: STR profile from personal communication of Rodriguez-Tarduchy Segovia, Gemma.
Derived from site: In situ; Peripheral nervous system; UBERON=UBERON_0000010.
Cell type: Neural crest derived neuroblast; CL=CL_0002676.
Disease Mouse neuroblastoma (NCIt: C21993)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: A/J.
Hierarchy Parent: CVCL_4343 (C-1300)
Children:
CVCL_B7VJ (Abcam Neuro-2a Arc KO) CVCL_6246 (AGN2a) CVCL_4510 (C1300 CLONE NA)
CVCL_C5RF (N2a #34) CVCL_C5RG (N2a #58) CVCL_ZM10 (N2A FLAG-Cdh1)
CVCL_A0TK (N2A Psen1/Psen2 dKO) CVCL_WI11 (N2a-R33) CVCL_WI08 (N2a/Gary)
CVCL_D649 (N2aEGFP) CVCL_D650 (N2aSW) CVCL_RW97 (Na 42/13)
CVCL_B436 (NB2a/d1) CVCL_DF49 (Neuro-2a NucLight Green) CVCL_DF50 (Neuro-2a NucLight Red)
CVCL_K046 (Neuro-2a-Luc) CVCL_C9FD (Neuro-2a/Cas9-Rosa26-Hyg) CVCL_C9FE (Neuro-2a/Cas9-Rosa26-Neo)
CVCL_C9FC (Neuro-2a/Cas9-Rosa26-Puro) CVCL_A9J5 (Neuro-2a/CMV-Luc) CVCL_C9FB (Neuro-2a/HF-Cas9-Rosa26)
CVCL_C9FF (Neuro-2a/LoxP-Cas9-hyg-Rosa26) CVCL_F275 (Neuro-2aTG) CVCL_D3XR (Neuro2a-RXR)
CVCL_E1GT (Neuro2A[TRb1]) CVCL_6268 (ScN2a) CVCL_E0ZD (Ubigene N2A Aldh2 KO)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): Direct_author_submission
Markers:
Mouse STR 1-1 11
Mouse STR 1-2 17,18
Mouse STR 2-1 16
Mouse STR 3-2 12,13,14
Mouse STR 4-2 21.3,22.3
Mouse STR 5-5 15,17
Mouse STR 6-4 18,20
Mouse STR 6-7 12
Mouse STR 7-1 25.2
Mouse STR 8-1 16,17
Mouse STR 11-2 15
Mouse STR 12-1 16
Mouse STR 13-1 16.2,17.2
Mouse STR 15-3 21.3,22.3,23.3
Mouse STR 17-2 16
Mouse STR 18-3 22,23
Mouse STR 19-2 12
Mouse STR X-1 26,27
Run an STR similarity search on this cell line
Publications
PubMed=11470893; DOI=10.1073/pnas.151242598; PMCID=PMC55414
Enari M., Flechsig E., Weissmann C.
Scrapie prion protein accumulation by scrapie-infected neuroblastoma cells abrogated by exposure to a prion protein antibody.
Proc. Natl. Acad. Sci. U.S.A. 98:9295-9299(2001)
PubMed=14504404; DOI=10.1073/pnas.1834432100; PMCID=PMC208815
Klohn P.-C., Stoltze L., Flechsig E., Enari M., Weissmann C.
A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions.
Proc. Natl. Acad. Sci. U.S.A. 100:11666-11671(2003)
PubMed=19903493; DOI=10.1016/j.jneumeth.2009.11.004
Tremblay R.G., Sikorska M., Sandhu J.K., Lanthier P., Ribecco-Lutkiewicz M., Bani-Yaghoub M.
Differentiation of mouse Neuro 2A cells into dopamine neurons.
J. Neurosci. Methods 186:60-67(2010)
PubMed=25193168; DOI=10.1074/mcp.M113.037291; PMCID=PMC4256493
Hornburg D., Drepper C., Butter F., Meissner F., Sendtner M., Mann M.
Deep proteomic evaluation of primary and cell line motoneuron disease models delineates major differences in neuronal characteristics.
Mol. Cell. Proteomics 13:3410-3420(2014)
PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. 3rd, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)
PubMed=25565633; DOI=10.1371/journal.pone.0117154; PMCID=PMC4286239
Oelschlegel A.M., Geissen M., Lenk M., Riebe R., Angermann M., Schaetzl H., Groschup M.H.
A bovine cell line that can be infected by natural sheep scrapie prions.
PLoS ONE 10:E0117154-E0117154(2015)
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
小鼠脑神经瘤细胞概述及培养方法！ 一、细胞介绍 克隆 Neuro-2A 是由 R.J.Klebe 和 F.H.Ruddle 经 A 株白鼠的自生肿瘤建立。该细胞产生大量微管蛋白，此蛋白在神经细胞中起应答轴浆流动的收缩系统作用。 二、基本信息 平台编号：bio-73109
实验：通过 CCK-8 分析确定 TMT 在 Neuro-2a 细胞中的神经毒性。用 2μM、4μM 和 8μM TMT 处理细胞 24 小时分别导致细胞活力降低约 15％，31％ 和 44％。 蛋白质组分析：确定了 8μM TMT 处理组和对照组的蛋白质表达谱。与对照组相比，TMT 组中共有 340 种差异蛋白，其中 204 种蛋白上调，136 种蛋白下调，进一步研究表明 TMT 破坏了自噬体的清除，导致它们在细胞质中的积累。IPA 显示出对 KIF5A 信号通路的抑制与自噬相关蛋白显著相关
Analysis of RB Action in DNA Damage Checkpoint Response
checkpoint response: (1) transcriptional repression of E2F-regulated genes (cyclin A reporter assay); (2) induction of cell cycle arrest (Brd-U incorporation assay); and (3) inhibition of DNA double-strand break accumulation (phosphorylated-histone H2A.X
技术资料
