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- 技术资料
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- 英文名:
pRL-CMV
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- 保质期:
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- 供应商:
武汉华尔纳生物科技有限公司
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Product Introduction
| 品牌 |
华纳生物 | ||||||||||||||||||
| 货号 |
WN-13326 | ||||||||||||||||||
| 规格 |
2ug | ||||||||||||||||||
| 价格 |
询价 | ||||||||||||||||||
| 货期 |
现货 | ||||||||||||||||||
基本信息
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质粒图谱![]() 载体描述The pRL-CMV Vector(a,b,c) (Figure 1) is intended for use as an internal control reporter and may be used in combination with any experimental reporter vector to co-transfect mammalian cells. All of Promega’s pRL Reporter Vectors contain a cDNA (Rluc)(a) encoding Renilla luciferase, which was originally cloned from the marine organism Renilla reniformis (sea pansy; 1). As described below, the Renilla luciferase cDNA contained within the pRL Vectors has been modified slightly to provide greater utility. The pRL-CMV Vector contains the CMV(b) enhancer and early promoter elements to provide high-level expression of Renilla luciferase in co-transfected mammalian cells. Renilla luciferase is a 36kDa monomeric protein that does not require posttranslational modification for activity (2). Therefore, like firefly luciferase, the enzyme may function as a genetic reporter immediately following translation. For information about the use of this plasmid in conjunction with a reporter vector containing the firefly luciferase gene, refer to the Dual-Luciferase® Reporter Assay System(c,d,e) Technical Manual (#TM040). To avoid DNA methylation, all pRL Vectors are isolated from a dam-/dcm- E. coli K host strain. Therefore, initial propagation of the pRL-CMV Vector should be conducted in an appropriate E. coli host strain lacking endogenous restriction endonuclease activity (e.g., JM109). |
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武汉华尔纳生物科技有限公司,简称华尔纳生物(Warner Bio),坐 落于武汉生物产业基地·光谷生物城,是一家生物医学资源开发,科研生 产,产品销售集一体的科技型企业。
华尔纳生物(Warner Bio)始终专注于生命科学领域及细胞实验培养 方向,坚持为客户打造优质高效,安全可靠的生命科学产品,我们始终坚 持于以客户为中心、以品质求生存、以价值求发展、以服务求口碑的企业 理念,从而获得全国广大科研用户的一致认可。
团队以高效的服务品质,专业卓越的技术能力构建了华中地区最大的 生物细胞资源典藏中心,经过多年不懈的努力与发展,现与武汉大学、华 中科技大学、武汉协和医院、武汉同济医院、四川大学华西医院、首都医 科大学、中山大学附属第三医院等全国多所高校、医院、研究所单位达成 长期稳定的合作,同时为科研人员提供免费专业的售前售中售后服务,为 您的科研实验保驾护航。
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