- 询价
- Biotium
- 美国
- 40044
- 2026年01月19日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
1年
- 英文名：
Hoechst 33258, 10mg/mL in H2O 40044
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
| Product | Catalog Number | MW | Unit Size | λEx/λEm | Format |
|---|---|---|---|---|---|
| Hoechst 33258, 10 mg/mL in Water | 40044 | 533.88 | 10 mL | 352/458 nm | Yellow solution |
| Hoechst 33258, pentahydrate | 40045 | 623.96 | 100 mg | 352/458 nm | Yellow solid |
| Hoechst 33342, 10 mg/mL in Water | 40046 | 561.93 | 10 mL | 350/461 nm | Yellow solution |
| Hoechst 33342, trihydrochloride trihydrate | 40047 | 615.98 | 100 mg | 350/461 nm | Yellow solid |
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Biotium offers Hoechst 33258 and Hoechst 33342 dyes in both solution or powder forms. Both dyes are spectrally similar but Hoechst 33528 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.
Hoechst can also be used to stain live bacteria (gram-positive and gram-negative), but in live yeast the staining is weak and not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Hoechst 33258, pentahydrate
Hoechst 33342, trihydrochloride trihydrate
Learn more about Hoechst and our other novel nuclear stains. We also offer DAPI nuclear stains in powder or solution.
Having trouble with your experiment? See our section on troubleshooting tips for fluorescent staining.
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文献和实验Testing for Mycoplasma by Indirect DNA Stain (Hoechst 33258 stain)
Materials Media� pre-warmed to 37ºC (refer to the ECACC Cell Line Data Sheet for the correct medium) 70% ethanol in water (Prod. No. R8382 ) Methanol (Prod. No. 175 ) Acetic Acid Glacial (Prod. No. A6283 ) Hoechst 33258
%乙醇中浸泡5分钟或更长时间，无菌超净台内吹干或用细胞培养PBS或0.9%NaCl等溶液洗涤三遍，再用细胞培养液洗涤一遍。将盖玻片置于六孔板内，种入细胞培养过夜，使约为50%-80%满。B. 刺激细胞发生凋亡后，吸尽培养液，加入0.5ml固定液，固定10分钟或更长时间（可4℃过夜）。C. 去固定液，用PBS或0.9%NaCl洗两遍，每次3分钟，吸尽液体。洗涤时宜用摇床，或手动晃动数次。D. 加入0.5ml Hoechst 33258染色液，染色5分钟。也宜用摇床，或手动晃动数次。E. 用PBS
颗粒位于细胞表面和细胞之间。 ②荧光染色法：荧光染色用能与DNA特异性结合的荧光染料Hoechst 33258，可使支原体内含有的DNA着色，染色后用荧光显微镜观察。具体方法如下：首先将细胞接种盖玻片上，在细胞未长满前取出玻片，用不合酚红的Hanks液漂洗一下，用l： 3醋酸甲酵固定10Min，然后用生理盐水漂洗。置于用生理盐水配浓度为50pg／ml的Hoechst 33258中染色10min。染色后用蒸溜水洗1—2min。向细胞面滴加pH5.5磷酸缓冲液数滴，然后置荧光显微镜下观察。镜下支原
