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文献和实验Identifying Acetylated Proteins in Mitosis
the acetylation status of several mitotic proteins by anti-acetyl-Lysine immunoprecipitation with a monoclonal antibody followed by Western blot analyses of the proteins of interest. We further confirmed by a reciprocal immunoprecipitation with protein-specific
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
control) relative to the level of the signal of interest (ChIP sample). This hampers distinguishing a ''true signal'' from the background signal. Therefore, we recommend setting up ChIP using an antibody against an invariant domain of histone H3 [19,20
TSA Treatment of Mammalian Cells
Introduction Prolonged treatment of proliferating mammalian cells with low doses of a histone deacetylase inhibitor, trichostatin A, specifically affects pericentric heterochromatin. Relocation of these regions towards the nuclear
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