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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG1, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
PE Fluorescent Dye
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
IF/ICC, FC (Intra)
- 靶点:
CD107b / LAMP2
- 抗体英文名:
PE Anti-Human CD107b / LAMP2 (H4B4)
- 抗体名:
PE Anti-Human CD107b / LAMP2 (H4B4)
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥930.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥2320.0 |
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 ul per 10^6 cells in a 100 µl suspension or 10 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-65053 targets CD107b / LAMP2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, FC (Intra) |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lysosomal-associated membrane protein 2 |
| 别名 | CD107b, LAMP2, LAMP-2, LAMP 2, H4B4 |
| 计算分子量 | 45 kDa |
| GenBank蛋白编号 | BC002965 |
| 基因名称 | LAMP2 |
| Gene ID (NCBI) | 3920 |
| ENSEMBL Gene ID | ENSG00000005893 |
| RRID | AB_2883850 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P13473 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).
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文献和实验| Species | Application | Title |
|---|---|---|
ACS Nano Hypoxia Reversion and STING Pathway Activation through Large Mesoporous Nanozyme for Near-Infrared-II Light Amplified Tumor Polymetallic-ImmunotherapyAuthors - Chang Qu |
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
Native Chromatin Preparation and Illumina/Solexa Library Construction
19, CD36, CD56, CD123, and glycophorin A. 9. Mix well and incubate for 10 min at 4°C-8°C or on ice. 10. Add 30 µL of T cell isolation buffer per 107 cells. 11. Add 20 µL of anti-biotin microbeads (from T
Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
3 CD19 B-cell lineage FITC, PE, APC, BIO
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