- ￥2800
- Proteintech
- CL594-66375
- 2025年10月13日
- WB, IF-P, FC (Intra)
- Mouse
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®594 Fluorescent Dye
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IF-P, FC (Intra)
- 靶点：
TUBB3-specific/TUJ1
- 抗体英文名：
CoraLite®594-conjugated TUBB3-specific/TUJ1 Monoclonal antibody
- 抗体名：
CoraLite®594-conjugated TUBB3-specific/TUJ1 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, PC-12 cells, Neuro-2a cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66375 targets TUBB3-specific/TUJ1 in WB, IHC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF-P, FC (Intra) |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tubulin, beta 3 |
| 别名 | TUBB3, TUBB3-specific, TUJ1, beta tubulin, Tubulin beta 3 chain |
| 计算分子量 | 55 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | NM_001197181 |
| 基因名称 | TUBB3 |
| Gene ID (NCBI) | 10381 |
| RRID | AB_2883571 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q13509 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IF | Sci Rep Sexual dimorphic impacts of systemic vincristine on lower urinary tract function.Authors - Nao Iguchi |
| mouse | IHC | Exp Cell Res A parasympathetic neurotransmitter induces myoepithelial cell differentiation during salivary gland development.Authors - Yuki Shindo |
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