- ￥3200
- Proteintech
- CL594-66065
- 2025年10月13日
- IF-P
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CD31 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®594 Fluorescent Dye
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
IF-P
- 靶点：
CD31
- 抗体英文名：
CoraLite®594-conjugated CD31 Monoclonal antibody
- 抗体名：
CoraLite®594-conjugated CD31 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66065 targets CD31 in WB, IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF-P |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag1787 Product name: Recombinant human CD31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 428-738 aa of BC022512 Sequence: EVRCESISGTLPISYQLLKTSKVLENSTKNSNDPAVFKDNPTEDVEYQCVADNCHSHAKMLSEVLRVKVIAPVDEVQISILSSKVVESGEDIVLQCAVNEGSGPITYKFYREKEGKPFYQMTSNATQAFWTKQKANKEQEGEYYCTAFNRANHASSVPRSKILTVRVILAPWKKGLIAVVIIGVIIALLIIAAKCYFLRKAKAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVGNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | platelet/endothelial cell adhesion molecule |
| 别名 | PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM |
| 计算分子量 | 83 kDa |
| GenBank蛋白编号 | BC022512 |
| 基因名称 | CD31 |
| Gene ID (NCBI) | 5175 |
| ENSEMBL Gene ID | ENSG00000261371 |
| RRID | AB_2883500 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P16284 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cell Metab Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunityAuthors - Zilong Zhang |
CD31 分子 CD31 常用单克隆抗体或代号： SG134,TM3,HEC- 75;(PECAM、EndoCAM) 主要表达细胞： Pt,M,G,B,NK,Tsub [AS] 分子质量(kDa)和结构： gp140,血小板GPⅡa(IgSF) 功 能： 嗜同性或嗜异性(与CD38互为受体)粘附,炎症,En功能 CD31 Aka
有好几个同仁发消息问CD31染色，刚工作比较忙，今天分享一下我做的是免疫荧光，也试做过免疫组化，都可以做出来抗体：小鼠抗CD31单克隆抗体 英国Abcam公司（CD31抗体说明书里面有说多聚甲醛会掩盖抗体，所以推荐速冻切片，也尝试过速冻切片，不使用多聚甲醛，但是这个技术个人掌握不好，所以还是用了多聚甲醛固定）二抗： 山羊抗小鼠488单克隆抗体 美国CST公司 特别说明：我是从同学那里得到的经验，可能绿光稍微好做些，之前做红光
Anti-Digoxin 产品详情 Dylight 594标记小鼠抗地高辛Digoxin单克隆抗体 Dylight 594-conjugated IgG Fraction Monoclonal Mouse Anti-Digoxin 产品详情
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