- ￥1350 - 3000
- Proteintech
- 28718-1-AP
- 2025年10月13日
- WB, IHC, ELISA
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human SMCR7,MID49 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, ELISA
- 靶点：
SMCR7/MID49
- 抗体英文名：
SMCR7/MID49 Polyclonal antibody
- 抗体名：
SMCR7/MID49 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28718-1-AP targets SMCR7/MID49 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse, pig, monkey |
| 免疫原 |
CatNo: Ag30119 Product name: Recombinant human SMCR7,MID49 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 283-454 aa of BC014973 Sequence: MASEELLLEVQHERLELTVAVLVAVPGVDADDRLLLAWPLEGLAGNLWLQDLYPVEAARLRALDDHDAGTRRRLLLLLCAVCRGCSALGQLGRGHLTQVVLRLGEDNVDWTEEALGERFLQALELLIGSLEQASLPCHFNPSVNLFSSLREEEIDDIGYALYSGLQEPEGLL |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Smith-Magenis syndrome chromosome region, candidate 7 |
| 别名 | MID49, SMCR7 |
| 计算分子量 | 454 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC014973 |
| 基因名称 | MID49 |
| Gene ID (NCBI) | 125170 |
| RRID | AB_2881198 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96C03 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.
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文献和实验| Species | Application | Title |
|---|---|---|
| pig | WB | Mol Med Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells.Authors - Leonard Quiring |
| mouse | WB | Cell Rep A PKD-MFF signaling axis couples mitochondrial fission to mitotic progression.Authors - Evanthia Pangou |
| human | WB | Nat Commun Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymesAuthors - Wenjing Yan |
| mouse | WB | Int J Biol Sci Mitochondrial Dysfunction by FADDosome Promotes Gastric Mucosal Injury in Portal Hypertensive GastropathyAuthors - Yuelin Xiao |
| human | WB | Technol Cancer Res Treat The Role of MIEF2 in Cisplatin Sensitivity in KIRP Patients: Insights from Four-gene Mitochondrial Fusion RNA MarkersAuthors - Yusong Hou |
| monkey | WB | PLoS Pathog Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferationAuthors - Zhe Sun
|
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
coupled with mass spectrometry (AP?MS) is a robust technique used to identify protein?protein interactions. With recent improvements in sample preparation, and dramatic advances in MS instrumentation speed and sensitivity, this technique
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
【原创】Tricine SDS-PAGE凝胶配方（16.5％）
，因此用的是NaOH，老外的文章也不可信哦），在加水定容到500ml。3.丙稀酰胺母液（49.5％T，6％C)Acrylamide 23.5g ， bis 1.5g 加50ml水4.凝胶缓冲液液（3X）：18.15g Tris，0.15gSDS，溶于40ml水中，用1mol/L的HCL滴定至PH8.45再加水至50ml。以上溶液均需millipore膜过滤。（millipore膜过滤，具体用多大规格的老外没说，我开始用的是0.25ul的，在后来重新配制的也都没过滤（忘了），感觉效果差不多，请战友自己在试验中感觉
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