- ￥1350 - 3000
- Proteintech
- 28273-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human MTOR protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
mTOR
- 抗体英文名：
mTOR Polyclonal antibody
- 抗体名：
mTOR Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, Rapamycin treated HEK-293T cells, HepG2 cells, MCF-7 cells, PC-3 cells, Rapamycin treated MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28273-1-AP targets mTOR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit, monkey, chicken, bovine |
| 免疫原 |
CatNo: Ag28395 Product name: Recombinant human MTOR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-291 aa of NM_004958 Sequence: MSGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQ |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FK506 binding protein 12-rapamycin associated protein 1 |
| 别名 | FRAP1, EC:2.7.11.1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2 |
| 计算分子量 | 289 kDa |
| 观测分子量 | 250-289 kDa |
| GenBank蛋白编号 | NM_004958 |
| 基因名称 | mTOR |
| Gene ID (NCBI) | 2475 |
| RRID | AB_2881102 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P42345 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).
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文献和实验| Species | Application | Title |
|---|---|---|
Brain Behav Immun l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.Authors - Danqing Xin | ||
| human | WB,IHC | J Adv Res Salidroside sensitizes Triple-negative breast cancer to ferroptosis by SCD1-mediated lipogenesis and NCOA4-mediated ferritinophagyAuthors - Guiqin Huang |
| mouse | WB | Proc Natl Acad Sci U S A Heterozygous LRP1 deficiency causes developmental dysplasia of the hip by impairing triradiate chondrocytes differentiation due to inhibition of autophagyAuthors - Wenjin Yan |
| human | WB | Phytomedicine Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cellsAuthors - Maolei Xu |
| human | WB | Free Radic Biol Med β-Sitosterol targets ASS1 for Nrf2 ubiquitin-dependent degradation, inducing ROS-mediated apoptosis via the PTEN/PI3K/AKT signaling pathway in ovarian cancerAuthors - Haoyu Wang |
| human | WB | J Transl Med Fasting mimicking diet inhibits tumor-associated macrophage survival and pro-tumor function in hypoxia: implications for combination therapy with anti-angiogenic agentAuthors - Lei Wang |
Rapid Inactivation of Proteins by Knocksideways
, the small molecule, the cell or organism, and the assay. Curr. Protoc. Cell Biol . 61:15.20.1?15.20.7. © 2013 by John Wiley & Sons, Inc. Keywords: clathrin; AP?1; AP?2; rapamycin; AP21967
和280KD都做出来了） 分离胶 积层胶 ddH2O 1.85 mL 2 mL AB-3 1 mL 250 uL Gel-Buffer(3*) 1.65 mL 750 uL 甘油 0.5 mL — 10%AP 25 uL 22.5 uL TEMED 2.5 uL 2.25 uL (这个配方来自DXY，今天又回来了，呵呵) 3*Gel
示意图 (A) 与 6-8 周龄雌性无胸腺裸鼠异种移植肿瘤生长情况 (B)[1]。 表观基因组重编程与转录因子转换是研究揭示的第一个关键机制。H3K27ac 作为活跃增强子/启动子的标志，其 ChIP-seq 分析显示，禁食联合 TMX 治疗导致了肿瘤表观基因组的广泛重编程。具体而言，禁食使 TMX 治疗下的肿瘤丧失了 289个 H3K27ac 富集区域，同时获得了 291 个新的 H3K27ac 位点。深入分析发现，丢失的 H3K27ac 位点显著富集于 AP-1 转录因子家族成员
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