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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human STX16 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, IHC, ELISA
- 靶点:
Syntaxin 16
- 抗体英文名:
Syntaxin 16 Polyclonal antibody
- 抗体名:
Syntaxin 16 Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11201-1-AP targets Syntaxin 16 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1657 Product name: Recombinant human STX16 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-304 aa of BC019042 Sequence: MATRRLTDAFLLLRNNSIQNRQLLAEQLADDRMALVSGISLDPEAAIGVTKRPPPKWVDGVDEIQYDVGRIKQKMKELASLHDKHLNRPTLDDSSEEEHAIEITTQEITQLFHRCQRAVQALPSRARACSEQEGRLLGNVVASLAQALQELSTSFRHAQSGYLKRMKNREERSQHFFDTSVPLMDDGDDNTLYHRGFTEDQLVLVEQNTLMVEEREREIRQIVQSISDLNEIFRDLGAMIVEQGTVLDRIDYNVEQSCIKTEDGLKQLHKAEQYQKKNRKMLVILILFVIIIVLIVVLVGVKSR |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syntaxin 16 |
| 别名 | Golgi Marker, hsyn16, STX16, SYN16, syntaxin 16 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC019042 |
| 基因名称 | Syntaxin 16 |
| Gene ID (NCBI) | 8675 |
| RRID | AB_2198362 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14662 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syntaxin 16 (STX16), also named as SYN16, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes. STX16 is localized to the Golgi apparatus.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Hum Mol Genet Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.Authors - Sean-Patrick Riechers |
| human | IF | Eur J Immunol SNARE protein expression and localization in human cytotoxic T lymphocytes.Authors - Pattu Varsha V |
| human | WB | Traffic Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.Authors - Gustaf E Rydell |
| human | WB | J Virol Potent Inhibition of HCMV by Modulating the Cellular SNARE Syntaxin 5.Authors - Linda Cruz |
| human | WB | Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.Authors - Nazma Malik |
Using ProHits to Store, Annotate, and Analyze Affinity Purification–Mass Spectrometry (AP‐MS) Data
of the software to analyze AP?MS data. Curr. Protoc. Bioinform. 39:8.16.1?8.16.32. © 2012 by John Wiley & Sons, Inc. Keywords: affinity purification coupled with mass spectrometry; data analysis; virtual machine; statistical models; protein
流速为14~16滴/min。分部收集器收集,紫外监测,或人工收集,以紫外分光光度计分别测定每管280nm OD值,描绘洗脱液峰。 6. 浓缩:将洗脱液的280nm OD上峰段与下峰段各管分别合并,装入透析袋,以PEG浓缩至所需体积。 三、Tris-PO4 离子交换层析法 该法在上述方法的基础上稍加改良,即通过梯度洗脱,可用于血清中IgG和IgM的提取。 材料和试剂 1. DE32或DE52纤维素。 2. 待纯化标本:杂交瘤腹水或培养上清,免疫血清或饱和硫酸铵粗提品。
【原创】Tricine SDS-PAGE凝胶配方(16.5%)
这篇配方是我原发在这儿的怕帖子沉了,搜索了一下就开了一个新主题。另有Tricine SDS-PAGE凝胶配方(10%)的配方,有战友需要话我再发出来 。希望用了这个配方的战友如果效果好的话请跟贴说明,效果不好的话,我尽可能帮助战友们分析一下问题,反正这是我实践过的东西,不应该跑步出来的呀^_^另外Tricine SDS-PAGE16.5%是我见过最浓的Tricine SDS-PAGE的胶了,真没听说过20%Tricine SDS-PAGE胶,如果谁有说一下效果怎么样,是否能分离到几百,甚至几十
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