- ￥1200 - 7200
- Proteintech
- HRP-66005
- 2025年10月13日
- WB
- Mouse
- recombinant protein
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
HRP
- 适应物种：
recombinant protein
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB
- 靶点：
6*His, His-Tag
- 抗体英文名：
HRP-conjugated 6*His, His-Tag Monoclonal antibody
- 抗体名：
HRP-conjugated 6*His, His-Tag Monoclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥1200.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4800.0 |
| 规格： | 1ml | 产品价格： | ￥7200.0 |
经过测试的应用
| Positive WB detected in | Recombinant protein protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-66005 targets 6*His, His-Tag in WB, IF, IP, CoIP, ELISA, Blocking assay, Cell treatment applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB |
| 文献引用应用 | WB, IF, IP, CoIP, ELISA, Blocking assay, Cell treatment |
| 经测试反应性 | recombinant protein |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | 6*His, His-Tag |
| 别名 | 6*His, 6*His, His Tag, 6*His, His-Tag, 6histag, 6XHis, HHHHHH, His tag, HISTIDINE TAGGED, HRP 6XHis, HRP HHHHHH, HRP His tag |
| 计算分子量 | 0.84 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2857904 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | HISTAG |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
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文献和实验| Species | Application | Title |
|---|---|---|
Cell Developing Covalent Protein Drugs via Proximity-Enabled Reactive Therapeutics.Authors - Qingke Li | ||
| WB | Immunity Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals.Authors - Ling Ni | |
| WB | Immunity Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.Authors - Yujia Wang | |
| CoIP | Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescenceAuthors - Yinsheng Wu | |
| WB | Nat Commun Antioxidant hepatic lipid metabolism can be promoted by orally administered inorganic nanoparticlesAuthors - Jie Cai | |
| WB | Nat Commun The structure of a Plasmodium vivax Tryptophan Rich Antigen domain suggests a lipid binding function for a pan-Plasmodium multi-gene familyAuthors - Prasun Kundu |
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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