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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG2b
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite®594 Fluorescent Dye
- 适应物种:
Megathura crenulata
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
IgG2b isotype control
- 抗体英文名:
CoraLite®594 Mouse IgG2b isotype control (11B8C4)
- 抗体名:
CoraLite®594 Mouse IgG2b isotype control (11B8C4)
- 规格:
25ug/100ug
| 规格: | 25ug | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1220.0 |
经过测试的应用
| Positive FC (Intra) detected in | |
| Positive FC detected in |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.4 ug per 10^6 cells in 100 μl suspension |
| Flow Cytometry (FC) | FC : |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66360-3 targets IgG2b isotype control in FC (Intra) applications and shows reactivity with Megathura crenulata samples.
| 经测试应用 | FC |
| 经测试反应性 | Megathura crenulata |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IgG2b isotype control |
| 别名 | Mouse IgG2b, Mouse IgG2b isotype control |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2883568 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
0.05-0.2 ug per test is suggested for FC experiment.
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
D Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A D8 IgG2b, 09821D Eryth: Ter-119 IgG2b, 09081A Sca-1 PE-IgG2a (E31-161.7) 01835B PE-IgG2a Isotype control, 11025
1:1000 Pharmingen 553142 FITC-IgG2b Isotype 1:100 Pharmingen 553988 PE-IgG2a Isotype 1:100 Pharmingen 553930 Mac-1 (CD11b
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