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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG1
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
recombinant protein
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
WB, IF/ICC, FC (Intra), IP, ELISA
- 靶点:
MYC tag
- 抗体英文名:
MYC tag Monoclonal antibody
- 抗体名:
MYC tag Monoclonal antibody
- 规格:
100ul/500ul/1ml
| 规格: | 100ul | 产品价格: | ¥980.0 |
|---|---|---|---|
| 规格: | 500ul | 产品价格: | ¥4000.0 |
| 规格: | 1ml | 产品价格: | ¥6000.0 |
经过测试的应用
| Positive WB detected in | Transfected HEK-293T cells, recombinant proein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60003-2-Ig targets MYC tag in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IF, IP, CoIP, ChIP |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human, pig |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Myc tag |
| 别名 | myc, EQKLISEEDL, MYC-tag |
| 基因名称 | Myc tag |
| Gene ID (NCBI) | 99 |
| RRID | AB_2734122 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | MYCTAG |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
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|---|---|---|
| WB | Nature Promotion of DNA end resection by BRCA1-BARD1 in homologous recombinationAuthors - Sameer Salunkhe | |
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6PAuthors - Yuan-Li Ai | ||
| WB | Cell Discov Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availabilityAuthors - Xinyu Zhang | |
| WB | Nat Cancer Lymphatic endothelial-like cells promote glioblastoma stem cell growth through cytokine-driven cholesterol metabolismAuthors - Linjie Zhao | |
| WB | Protein Cell BiFC and FACS-based CRISPR screening revealed that QKI promotes PABPN1 LLPS in colorectal cancer cellsAuthors - Mengxia Li | |
| CoIP | Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescenceAuthors - Yinsheng Wu |
大阪大学蛋白质研究所藤井勇树、高木淳一东北大学大学院医学系研究科金子美华、加藤幸成 前言在分析蛋白质的功能时最重要的是如何得到高纯度的蛋白。现今提纯蛋白最常用的方法是亲和标签系统。被统称为「Epitope Tag」的肽标签以及其单克隆抗体组成的系统有FLAG、HA、Myc等多种标签。但是,每个标签系统都有优缺点,并不存在完美的标签系统。于是,在克服了众多难关后我们终于开发出有超高亲和性的新型亲和标签系统“PA Tag System”1)。 PA tag的起源Epitope Tag
抗体的检测外,还应该用与其抗原成分相关的其它抗原进行交叉试验,方法可用ELISA、IFA法。例如:①制备抗黑色素瘤细胞的McAb,除用黑色素瘤细胞反应外,还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应,以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体,应首先考虑是否与表达菌株的蛋白有交叉反应,其次是与其它细胞因子间有无交叉。 2.McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
-CAM-L1、MAG、Po、F3、NILE、TAG1等以及CD22、CD48、α1-BgP分子等。某此IgH链中C H 2和C H 3可有8个β折叠,在这种情况下,其中一个β折叠股只有3个氨基酸残基。 在V、C1和C2三组中,有些Ig超家族成员之间的同源性要大于该组中平均的类似程度,如在V组中IgV、TCR-V、和CD8V结构域;C1组中IgC、TCR-C、MHC-C结构域;C2组中CD2和LFA-3,NCAM、NCAM-L1和MAG
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