- ￥980 - 6000
- Proteintech
- 10284-1-AP
- 2025年10月13日
- WB, IHC, IF-P, ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, ELISA
- 靶点：
Human IgG
- 抗体英文名：
Human IgG Polyclonal antibody
- 抗体名：
Human IgG Polyclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 规格： | 1ml | 产品价格： | ￥6000.0 |
经过测试的应用
| Positive WB detected in | human plasma tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10284-1-AP targets Human IgG in WB, IHC, IF-P, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA |
| 文献引用应用 | WB, IF, IP, CoIP, ChIP, RIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Human IgG |
| 别名 | |
| 计算分子量 | 155 kDa |
| 观测分子量 | 25 kDa, 50 kDa |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2877729 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This rabbit poly clonal antibody recognizes the human IgG.
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文献和实验| Species | Application | Title |
|---|---|---|
| WB | Sci Adv Therapeutic strategies targeting uPAR potentiate anti-PD-1 efficacy in diffuse-type gastric cancer.Authors - Long Qin | |
Int Immunopharmacol LncRNA-H19 induces hepatic stellate cell activation via upregulating alcohol dehydrogenase III-mediated retinoic acid signals.Authors - Zhi-Min Wang | ||
| WB | Arch Biochem Biophys Fructose-derived glycation and immune function: Effects on antigen binding in human IgG and lymphocytesAuthors - Feryal Akay | |
Front Bioeng Biotechnol Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes.Authors - Rui-Rui Guo | ||
Biosensors (Basel) Compact Surface Plasmon Resonance IgG Sensor Based on H-Shaped Optical Fiber.Authors - Yijian Huang | ||
| CoIP | Int J Oral Sci FAM64A promotes HNSCC tumorigenesis by mediating transcriptional autoregulation of FOXM1.Authors - Xinyuan Zhao |
Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
The quantification of human immunoglobulin G (IgG) and recombinant derivatives is required for many applications in research and diagnostics. Particularly in research, robust and simple protocols are necessary to determine the concentration
Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System
of the Baculovirus systems allows a rapid cloning and production timeline. The following protocol describes a Baculovirus Expression System for the Production of Recombinant Human IgG Antibodies.
实验原理 免疫电泳又称为γ球蛋白电泳或免疫球蛋白电泳技术，这是一种能够判断血液中三种免疫球蛋白IgM，IgG，IgA含量水平的实验方法。在这项实验技术中，首先利用蛋白质的分子量和所带电荷的比值运用水平琼脂糖凝胶电泳技术将蛋白质进行分离，然后将特异性的抗体引入到与电泳方向平行的凹槽中，在抗原和抗体的扩散过程中，抗原和抗体适当比例的结合会导致沉淀的产生。0.85%盐不仅可以终止扩散过程也可用于洗去未结合的蛋白，抗原和抗体结合所形成
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