- ￥980 - 6000
- Proteintech
- 10000-0-AP
- 2025年10月13日
- WB, ELISA
- Rabbit
- recombinant protein
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
recombinant protein
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, ELISA
- 靶点：
GST Tag
- 抗体英文名：
GST Tag Polyclonal antibody
- 抗体名：
GST Tag Polyclonal antibody
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4000.0 |
| 规格： | 1ml | 产品价格： | ￥6000.0 |
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10000-0-AP targets GST Tag in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB, IF, IP, CoIP, ChIP, ELISA |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GST Tag |
| 别名 | Glutathione S-Transferase, GST |
| 计算分子量 | 26 kDa |
| GenBank蛋白编号 | FN315687 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_11042316 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| IP | Cell The WDR11 complex is a receptor for acidic-cluster-containing cargo proteinsAuthors - Huaqing Deng | |
| WB,IP | Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesisAuthors - Saisai Guo | |
| WB | Gastroenterology PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesisAuthors - Tian-Yi Jiang | |
Ann Rheum Dis Autoantibodies identify primary Sjögren's syndrome in patients lacking serum IgG specific for Ro/SS-A and La/SS-BAuthors - Sherri Longobardi | ||
| WB | Immunity Targeting 7-Dehydrocholesterol Reductase Integrates Cholesterol Metabolism and IRF3 Activation to Eliminate Infection.Authors - Jun Xiao | |
| WB | Nat Cell Biol O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteinsAuthors - Yulin Chen |
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
先介绍一下His Tag，为5-15 His 串在一起的小肽， 在表达纯化中用的较为普遍的一种Tag, 从本质上说它是一种亲和性Tag，但也个案证明其也能做一些兼职工作：增加蛋白可溶性，提高蛋白稳定性。但它的本质工作是与IMAC亲和，使纯化过程变的更加程序化，更具可控性。 相对与其他亲和Tag,如 GST, Flag, Strep, 它的特点可谓鲜明： 一，小，浓缩的都是精华，6个His 放在一起约2.5KD大小。His 的等电点为7.6，在常用的纯化缓冲体系（pH 8.0）中
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
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