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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
GFP-Trap® Agarose, kit
- 保存条件：
Upon receipt store at +4°C. Do not freeze
- 规格：
20rxns
Product Information
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. The ChromoTek GFP-Trap® Agarose is a ready-to-use reagent.
| Description | The GFP-Trap Agarose Kit contains GFP-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of GFP-fusion proteins and their interacting factors. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains on the SDS-PAGE • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 25-30 µg of recombinant GFP per 25 µL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 1 pM |
| Compatibility with mass spectrometry | The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631357 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Kit components
| COMPONENT | DESCRIPTION |
|---|---|
| GFP-Trap® Agarose | 20 reactions (500 µL) |
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |
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文献和实验Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) generating a so called GFP-Trap. A direct comparison of the GFP-Trap with conventional antibodies for immunoprecipitation of GFP from crude cell lysates reveal that the GFP-Trap allows a very fast (~ 5 � 30 min) depletion of GFP from tested samples
green快速染料标记1 × 104个TYK-nu细胞，96孔板放置4h，然后加入2 × 104个或5 × 104个T细胞和激活剂scDb（anti-CD3ε scFv，单链双特异性抗体形式）进行共培养。不断减少的绿色荧光信号，表明激活的T细胞具有显著的免疫杀伤作用。同时，通过Incucyte®系统的10×物镜连续拍摄120h，计算每孔中每mm2的gfp阳性对象数量，发现H2-scDb介导的细胞毒性同样通过TP53的破坏而减轻。 2. Nature：神经细胞吞噬及分化 应用领域：神经
针对同一指标，生化试剂盒和 ELISA 试剂盒的检测结果趋势是一致的吗？
的特异性产生是由于抗原决定簇决定，抗体产生的过程也比较复杂，特别是小分子抗原必须偶联到载体蛋白上才能产生抗体。 ELISA测定结果的表现形式: 物质浓度：单位主要是μg/mL。 二、从微观角度来看 某一物质的抗原决定簇的活性位点(或者抗原抗体特异性结合位点)，与化学反应的活性位点可能存在差异。 三、总的来看 针对同一指标，生化试剂盒和ELISA试剂盒的检测结果趋势是正相关、负相关还是不相关的问题，需要更高技术设备和方法，以及大量的实验数据来验证。从原理中也说明了ELISA试剂盒分种属，而生化试剂盒
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