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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
PE/Cyanine5 Anti-Mouse CD335/NKp46 Antibody流式抗体[29A1.4]_货号:E-AB-F1182G
- 抗体英文名:
PE/Cyanine5 Anti-Mouse CD335/NKp46 Antibody[29A1.4]_货号:E-AB-F1182G
- 应用范围:
FCM
- 宿主:
Rat
- 适应物种:
Mouse
- 保质期:
12个月
- 目录编号:
E-AB-F1182G
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
PE/Cyanine5
- 克隆性:
Monoclonal
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态:
液体
- 亚型:
Rat IgG2a, κ
- 规格:
100Tests/50Tests/100Tests×2
| 规格: | 100Tests | 产品价格: | ¥3420.0 |
|---|---|---|---|
| 规格: | 50Tests | 产品价格: | ¥2160.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥5100.0 |
货号:E-AB-F1182G
| Background |
CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.
|
| Alternate Names |
Ly94;mAR-1;NK-p46;mNKp46;Lymphocyte antigen 94;
|
| Swissprot |
Q8C567
|
| Gene ID |
17086
|
| Clone No |
29A1.4
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2a, κ
|
| Isotype Control |
PE/Cyanine5 Rat IgG2a, κ Isotype Control[2A3]
|
| Conjugation |
PE/Cyanine5
|
| Conjugation Information |
PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
从单个小鼠肿瘤样本中分选高纯度、高活性 TIL 细胞亚群的完整工作流程
分选仪可实现从同一肿瘤样本中连续分离高纯度和高细胞活力的 TIL 细胞的两个亚群:肿瘤浸润性 NK 细胞和肿瘤浸润性 B 细胞。 2.1 肿瘤浸润性 NK 细胞分选 经过预富集所得到的 CD45+ TIL 细胞通过第一轮的分选,可得到纯度高于 96% 且细胞存活率高于 98% 的肿瘤浸润性 NK 细胞 (CD45+/CD11b–/Anti-NKp46+, 见图 B)。 2.2 肿瘤浸润性 B 细胞分选 以第一轮分选剩余的非目标细胞 (即非肿瘤浸润性 NK 细胞) 作为起始样本进行的第二轮分选 , 可得
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
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