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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Phospho-AMPK alpha1/2 (Thr183/172) 多克隆抗体_货号:E-AB-21121_修饰性抗体
- 抗体英文名:
Phospho-AMPK alpha1/2 (Thr183/172) 多克隆抗体
- 宿主:
Rabbit
- 适应物种:
Human;Mouse;Rat;Monkey
- 目录编号:
E-AB-21121
- 级别:
科研级别
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
在-20℃下储存有效期为12个月。 避免冻融循环。
- 形态:
液体
- 亚型:
IgG
- 规格:
120μL/60μL/20μL
| 规格: | 120μL | 产品价格: | ¥2415.0 |
|---|---|---|---|
| 规格: | 60μL | 产品价格: | ¥1510.0 |
| 规格: | 20μL | 产品价格: | ¥630.0 |

Phospho-AMPK alpha1/2 (Thr183/172) 多克隆抗体产品名称:Phospho-AMPK alpha1/2 (Thr183/172) 多克隆抗体, 货号:E-AB-21121
| 已验证样本 | Western Blot(WB)已验证样本:L929,Rat kidney,Mouse brain 免疫组织化学(IHC)已验证样本:Mouse brain 免疫荧光(IF)已验证样本:Rat heart |
| 稀释 | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000 |
| 克隆性 | Polyclonal |
| 同种型 | IgG |
| 免疫原 | Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172 |
| Abbre | AMPKα1/2 (phospho Thr183/172) |
| 同义词 | PRKAA1;AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;Acetyl-CoA carboxylase kinase;ACACA kinase;Hydroxymethylglutaryl-CoA reductase kinase;HMGCR kinase;Tau-protein kinase PRKAA1;PRKAA2;AMPK;抗体 |
| Swissprot | Q13131,P54646 |
| 计算的MW | 62kDa |
| 实测MW | 63kDa |
| 细胞定位 | Cytosol,Nucleus,nuclear speck,nucleoplasm,nucleus,Plasma Membrane,apical plasma membrane,Other locations:cytoplasm,intracellular,nucleotide-activated protein kinase complex |
| 浓度 | 1 mg/mL |
| 储存方法 | 在-20℃下储存有效期为12个月。 避免冻融循环。 |
| 缓冲 | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
| 纯化方法 | Affinity purification |
| 研究领域 | Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction |
| Conjugation | Unconjugated |
| 产品描述: | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. |

Phospho-AMPK alpha1/2 (Thr183/172) 多克隆抗体Elabscience®流式抗体致力于为您的流式细胞分析实验带来更优质的体验,已有3,000+指标,选用经典克隆株纯化抗体,产品涵盖抗人/大鼠/小鼠等,指标丰富。

Phospho-AMPK alpha1/2 (Thr183/172) 多克隆抗体在-20℃下储存有效期为12个月。 避免冻融循环。
Phospho-AMPK alpha1/2 (Thr183/172) 多克隆抗体产品更多产品信息查看欢迎咨询我们和搜索武汉伊莱瑞特Elabscience官网查看。
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内,每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激,我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤,分别固定,打孔,猝灭,封闭,然后一抗孵育,二抗孵育,最后显色,用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正,最后得出以上数据。Quantitative
位升高),不知道我的这个结果是否可以说明gsk活性降低了?还是一定需要第九位的结果才能解释这个问题?似乎也有些人说216特异性不强,没有第九位有说服力,不是很能理解这其中的原因,再次感谢你! ylhuang0502 As for the GSK phosphorylation, it is clear now that, GSK serine phosphorylation (S9 for beta and S21 for alpha
-l1-antibody-ya4674.html 兔来源、无偶联标记、抗 PD-L1 的 IgG 单克隆抗体。 Estrogen Receptor alpha Antibody (YA450) (HY-P80663) www.medchemexpress.cn/antibody/estrogen-receptor-alpha-rabbit-mab.html 兔来源、无偶联标记、抗 Estrogen Receptor alpha 的 IgG 单克隆抗体 CDX2 Antibody (YA3498
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