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上海臻诺生物科技有限公司
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文献和实验complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl
Pol III whole cell yeast extract (small scale)
, transfer tube to ice and add 15% the total volume of extraction buffer (i.e 5 g cells + 750 ul buffer) and let sit on ice 20 min. Scrape the yeast sorbet into an ultraclear SW55 tube and spin in ultracentrifuge 100,000 x g for 1 hr at 4 deg. (33,000 rpm
SOLID TUMOR CULTURES FOR CHROMOSOME ANALYSIS
free, 500 ml bottle. Nutrient Mix F-10: GIBCO cat#430-1200. Nutrient Mixture F-10 (HAM) powdered form, prepared to instructions, 10 x 1 liter package. Penicillin/Streptomycin: GIBCO cat# 600-5140. Penicillin/Streptomycin solution, 10,000
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