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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4°C
- 保质期:
12个月
- 英文名:
Thiazole Green, 10,000X in DMSO 40086-1mL
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
Thiazole Green, is structurally identical to the well known qPCR and DNA gel stain SYBR® Green I. Thiazole Green is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR. Thiazole Green can also be used to detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any prewashing steps, although the sensitivity is lower. Thiazole Green may also be used as a green nuclear stain for all cells in live cultures, but will lose nuclear specificity after fixation. Thiazole Green has the identical spectral properties as SYBR® Green I (Ex/Em 498/522 nm with DNA).
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文献和实验Vybrant® DyeCycle Green and Orange Stains
add 2 μL of Vybrant® DyeCycle™ Green stain or Vybrant® DyeCycle™ Orange stain. Final stain concentration is 10 μM. After use, seal the stain vial tightly. The stain in DMSO solution may be subjected to many freeze-thaw cycles without reagent
SUPER Green Ⅰ(10,000× DMSO溶液,电泳级) SUPER Green Ⅰ核酸染料特点 ● 无毒性:属花箐类染料,容易生物降解,无致癌毒性。 ● 灵敏度高:至少可检出20pg DNA,高于EB染色法25~100倍。 ● 信噪比高:样品荧光信号强,背景信号低。 ● 操作简单:无须脱色或冲洗,即可直接用紫外凝胶透射仪或可见光透射仪观察。 ● 适用范围广:可适用于多种凝胶
Staining Methods for cell death Z. Xia 10/2/95
The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead cells 35 mm plates: to 2 ml medium or PBS, add 2 ul 2 mg/ml P












