- 询价
- Biotium
- 美国
- 92430
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
Mix-n-Stain™ antibody labeling kits CF®800 dyes,50-100 ug labeling 92430
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification.
Features
- Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
- Choice of 32 CF® Dye colors
- Less than 30 seconds of hands-on time
- 30 minutes total reaction time
- No purification, 100% recovery
- Compatible with BSA, gelatin, ascites
Kit components
- Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
- 10X Mix-n-Stain™ Reaction Buffer
- Lyophilized Reactive CF® Dye
- Mix-n-Stain™ Storage Buffer
Unrivaled Convenience
Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.
Superior CF® Dyes
These kits feature a choice of 32 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Choose the Right Labeling Kit for Your Antibody
Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.
See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye, or see our CF® Dye Brochure for more detailed dye information.
Other Labeling Kits
We now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with enzymes (HRP, AP, and GOx), fluorescent proteins (PE, APC, PerCP, and tandem dyes), biotin, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.
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文献和实验Revised amino-allyl labeling protocol
and the Howard Hughes Medical Institute http://cmgm.stanford.edu/pbrown/protocols/amino-allyl.htm A. RT Reaction 1. To anneal primer, mix 1-2 m g mRNA with 5 ug of anchored oligo-dT [(dT)20 -VN] (Operon, HPLC purified) in a total volume of 18 m L
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
5 Labeling of RNA for Microarrays #2 Modified to use Genisphere Sub-micro kit **** CAUTION *** Protocol under construction! In microfuge tube combine: ("Primer Mix", 10ul) 2ul total RNA (~.5 ug) 3ul RT Primer (0.2 pmole… "Vial 2") 5ul RF-H2
We typically use 0.5ug of E. coli genomic DNA in a labeling reaction for each hybridization. The genomic DNA was fragmented to 500 to 1000bps before labeling. The following protocol should produce enough labeled probe for 8 hybridizations
