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One-Step 羽扇豆素蛋白凝胶染色, 1X 21004-

4L
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  • 询价
  • Biotium已认证
  • 美国
  • 21004-4L
  • 2025年07月16日
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    2金牌会员
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      4°C

    • 保质期

      6个月

    • 英文名

      One-Step Lumitein Protein Gel Stain, 1X 21004-4L

    • 库存

      99

    • 供应商

      Biotium

    • CAS号

      1

    One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent red protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain in terms of convenience and safety. One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band.

    • One-step staining procedure, no fixation required
    • Non-toxic formulation is safe for easy disposal
    • Sensitive protein detection down to 1-10 ng
    • Image with a UV transilluminator, a blue light transilluminator, or laser-based gel scanner
    • 1 L and 4 L sizes
    • Store at 4°C

    Fast and simple procedure

    One-Step Lumitein™ gel staining requires only a single 5-60 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Staining can be imaged using a UV transilluminator with EtBr or SYBR filter set, a blue light transilluminator, or a laser-based gel scanner.

    One-Step Lumitein™ is non-toxic

    One-Step Lumitein™ Protein Gel Stain offers safer handling and ease of disposal compared to other protein gel stains, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.

    产品细节图片1
    Red fluorescent One-Step Lumitein™ is an alternative to time-consuming and expensive SYPRO® Ruby protein gel stain, and it can be imaged on a UV box or fluorescence gel scanner

    Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ UV, which is optimized for use with a UV transilluminator, and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.

    Still deciding? Request a free sample of any of our One-Step protein gel stains.

     

    Request a Free Sample

     

    Product Replacement for Catalog number Unit size Product protocol Safety report
    One-Step Blue® Coomassie stain 21003-1L 1L PI-21003 One-Step Gel Stains Safety Report
    One-Step Lumitein™ SYPRO® Ruby Protein Gel Stain 21004-1L 1L PI-21004
    21004-4L 4L
    One-Step Lumitein™ UV Oriole™ and Flamingo™ Protein Gel Stains 21005-1L 1L PI-21005
    21005-4L 4L
    One-Step Blue is a registered trademark of Biotium. Lumitein and its related technologies are covered by US and international patents. SYPRO is a registered trademark of Molecular Probes, Inc. Oriole and Flamingo are trademarks of Bio-Rad Laboratories. Cubitainer is a registered trademark of Hedwin Corporation.

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    图标文献和实验
    相关实验
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      2 ( 25 mM ) 0.5 ul  3.1.2.5 Taq DNA polymerase ( 5 U/ul ) 0.1 ul  3.1.2.6 ddH2O 18.4 ul  3.1.3 PCR program ( 1st PCR与2 nd PCR相同):使用PCR thermal cycler  3.1.3.1 step 1 : denaturation: 94 ℃ 30 sec  3.1.3.2 step 2 : denaturation: 94 ℃ 30 sec  3.1.3.3 step

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      2006 ). Suspend the DNA in a total volume of 25 µL of 0.1X TE-Ad.   12. Repeat Steps 5-10 with 5 µL of DNA (from Step 11) and 100 µL of DH5 cells, rather than BJ5183.   13. Select two colonies from each plate. Purify the DNA

    图标技术资料

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    资料下载:

    PI-21004.pdf 附 (下载 0 次)

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