- 询价
- Cell Signaling Technology
- 3359
- USA
- 2025年11月22日
- W
- Rabbit
- H,M,R
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Torc1/Crtc1 (Ser151) Antibody
- 抗原：
synthetic phosphopeptide corresponding to the sequence of human Torc1/Crtc1
- 应用范围：
W
- 宿主：
Rabbit
- 保质期：
详见说明书
- 库存：
大量
- 适应物种：
H,M,R
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) | Transfected Only | 82 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Torc1/Crtc1 (Ser151) Antibody detects transfected levels of Torc1/Crtc1 protein when phosphorylated on Ser151. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of human Torc1/Crtc1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from U-87 MG cells, untransfected or transfected with human wild-type (WT) Torc1 or Torc1 (Ser151Ala), using Phospho-Torc1/Crtc1 (Ser151) Antibody (upper) or DYKDDDDK Tag Antibody (Binds to the same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower). DYKDDDDK-tagged WT and mutant Torc1 expression vectors were kindly provided by Dr. Marc Montminy at the Salk Institute for Biological Studies. |
| Background | Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling (1). The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator (2,3) and is implicated in mediating the effects of these two pathways (4). In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting (5). Torc2-related proteins Torc1 and Torc3 also act as CREB co-activators (2,3). Torc1, Torc2 and Torc3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats (6,7). Torc1 is highly phosphorylated at Ser151 in mouse hypothalamic cells under basal conditions (8). When these cells are exposed to cAMP or a calcium activator, Torc1 is dephosphorylated and translocates into the nucleus (8). Torc1 is essential for energy balance and fertility (8).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Anti-FLAG® is a registered trademark of Sigma-Aldrich. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
双特异抗体(Bispecific antibody)的简介和制作方法
contain two identical antigen-binding arms and a constant fragment, (Fc)g. The Fc part enables the antibody to function as an adaptor protein, linking antibody-bound cells to immune cells bearing Fcg receptors. Because there are different Fcg receptors
XBB.1.5 毒株的传播力为何这么强？北大曹云龙团队最新研究揭示相关机制
1 月 3 日，北京大学曹云龙团队及其合作者在预印本平台 bioRxiv，发表了题为 Enhanced transmissibility of XBB.1.5 is contributed by both strong ACE2 binding and antibody evasion 的文章，报道了关于 XBB.1.5 的最新研究成果。该研究表明，XBB.1.5 的传播性增强是由与 ACE2 的强结合和抗体逃逸共同促成的，该团队此前开发的中和抗体 SA55 对 XBB.1.5 仍有很强的活性
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