- 询价
- Cell Signaling Technology
- 4953
- USA
- 2025年08月15日
- W, IP, IHC-P, IF-IC, F
- Rabbit
- H,M,R,Mk,C
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
PP2A B Subunit Antibody
- 抗原：
synthetic peptide corresponding to amino acids near the amino terminus of human PP2A B subunit
- 应用范围：
W, IP, IHC-P, IF-IC, F
- 宿主：
Rabbit
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H,M,R,Mk,C
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk (C) | Endogenous | 52 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | PP2A B Subunit Antibody detects endogenous levels of the PR55 PP2A B subunit (α isoform). The antibody may also recognize the β, γ, and δ isoforms of the PR55 PP2A B subunit. This antibody does not cross-react with the B-prime (PR61), B-prime-prime, or B-prime-prime-prime families of PP2A B subunits. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human PP2A B subunit. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells using PP2A B Subunit Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PP2A B Subunit Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using PP2A B Subunit Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PP2A B Subunit Antibody. Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells using PP2A B Subunit Antibody (blue) compared to a nonspecifc negative control antibody (red). IF-IC
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells using PP2A B Subunit Antibody. |
| Background | Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B'' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport
Shiga toxin, produced by Shigella dysenteriae and Shiga toxin-producing Escherichia coli , is a bacterial protein toxin composed of two subunits, A and B (1 ). The A-subunit is the actual toxin whose ribosomal RNA N -glycosidase activity
Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry
meningococci, there seems to be a correlation between SBA activity and protection (1 ), whereas OP has been less well-studied. This function may have a supplementary or even major role in protection against group B meningococci.
Buffer and save the flowthrough.(12) Wash with 6 ml Buffer A, 6 ml Buffer B, and 6 ml Buffer A. Hold at room temperature for 30 minutes.(13) Wash with 6 ml Buffer B, 6 ml Buffer A, and 6 ml Buffer B. Wash with Storage Buffer and hold at 4°C.(14) To purify
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