- 询价
- Cell Signaling Technology
- 2211
- USA
- 2025年11月03日
- W, IP, IHC-P, IHC-F, IF-IC, F
- Rabbit
- H,M,R,Mk,Sc,C,X
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6
- 应用范围：
W, IP, IHC-P, IHC-F, IF-IC, F
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,R,Mk,Sc,C,X
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken X=Xenopus Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IHC-F IF-IC F | H M R Mk Sc (C) (X) | Endogenous | 32 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-S6 Ribosomal Protein (Ser235/236) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236. This antibody does not detect ribosomal protein S6 phosphorylated at other sites. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indiccated time, using , Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (upper) or Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (lower). IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human melanoma, showing cytoplasmic localization of phosphorylated S6 ribosomal protein, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or calf-intestinal phosphatase (CIP) treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Imunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody preincubated with control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right). IHC-F (frozen)
Immunohistochemical analysis of frozen U87MG xenograft, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, U0126- and Rapamycin-treated (blue) or serum-treated (green), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody compared to a nonspecific negative control antibody (red). IF-IC
Immunofluorescent analysis of HeLa cells, untreated (A), 20% serum-treated (B) and serum-treated after rapamycin pretreatment (C), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IF-IC
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or 20% serum-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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