- 询价
- Cell Signaling Technology
- 2211
- USA
- 2025年11月03日
- W, IP, IHC-P, IHC-F, IF-IC, F
- Rabbit
- H,M,R,Mk,Sc,C,X
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6
- 应用范围：
W, IP, IHC-P, IHC-F, IF-IC, F
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,R,Mk,Sc,C,X
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken X=Xenopus Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IHC-F IF-IC F | H M R Mk Sc (C) (X) | Endogenous | 32 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-S6 Ribosomal Protein (Ser235/236) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 and 236. This antibody does not detect ribosomal protein S6 phosphorylated at other sites. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indiccated time, using , Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (upper) or Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (lower). IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human melanoma, showing cytoplasmic localization of phosphorylated S6 ribosomal protein, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or calf-intestinal phosphatase (CIP) treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Imunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody preincubated with control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right). IHC-F (frozen)
Immunohistochemical analysis of frozen U87MG xenograft, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, U0126- and Rapamycin-treated (blue) or serum-treated (green), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody compared to a nonspecific negative control antibody (red). IF-IC
Immunofluorescent analysis of HeLa cells, untreated (A), 20% serum-treated (B) and serum-treated after rapamycin pretreatment (C), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody. IF-IC
Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or 20% serum-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验构成核糖体的蛋白质。大肠杆菌核糖体蛋白的初级结构均被确定。大肠杆菌核糖体的 30S亚基含 S1 — S21 共 21种蛋白质， 50S亚基含 L1— L34共 34种蛋白质。这些蛋白质已被全部分离纯化。分子量约 1万到 3万。除 S6 、 L7 、 L12 之外全是碱性蛋白质。这些蛋白质是免疫学上独立的蛋白质，只有 L7 、 L1 2 显示出相互交叉反应。已知 L7 与 L1 2 是同一蛋白质， L7 的 N末端被乙酰化。已经确定了几种蛋白的一级结构。机能已经
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
: phospho?motif antibody; phosphorylation; protein kinase GO TO THE FULL PROTOCOL: PDF or HTML at Wiley Online Library
Optimized Protocol to Make Phospho-Specific Antibodies that Work
will describe the development of a revolutionary immunochemical technique that produces antibodies that bind to target proteins only when the protein is in the phosphorylated state. These phospho-specific antibodies can thus be used to track the activity of a protein
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