- 询价
- Cell Signaling Technology
- 2776
- USA
- 2025年10月28日
- W, IP
- Rabbit
- H,R,M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-PEA-15 (Ser104) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser104 of human PEA-15
- 应用范围：
W, IP
- 宿主：
Rabbit
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 适应物种：
H,R,M
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H R (M) | Endogenous | 15 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-PEA-15 (Ser104) Antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser104 of human PEA-15. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from serum starved human A172 and rat C6 cells, untreated or treated with 200 nM TPA, using Phospho-PEA-15 (Ser104) Antibody (upper) or PEA-15 Antibody (lower). |
| Background | PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as in tissues, including the lung and eye (1). The protein has been shown to coordinate cell growth, death, and glucose ultilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and futher regulates the Erk and apoptosis signaling pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
., Finstad, J., Williams, Jr., R. C. 1970. Phylogenetic insight into evolution of mammalian Fc fragment of g G globulin using staphylococcal protein A. J. Immunol. 104:140-147. Kronvall, G. 1973. A surface component in group A, C, and G streptococci
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
and stimulated with 0, 0.1, 1 and 10 µg/ml anisomycin for 15 minutes (Lanes 1–4). A Western blot was performed with 8 mg of extracts using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa assays were performed directly in the 96-well plates
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